Detailed information of TA system TA235274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 735431..736007
• Replicon: chromosome
• Accession: NZ_CP091928
• Organism: Leptospira noguchii strain IP1712055

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: -
• Locus tag: MAL02_RS02880
• Protein ID: WP_002155521.1
• Coordinates: 735431..735772 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: M6U786
• Locus tag: MAL02_RS02885
• Protein ID: WP_004425715.1
• Coordinates: 735759..736007 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAL02_RS02870 (MAL02_02870)	730750..731028	-	279	WP_002155541.1	GNAT family N-acetyltransferase	-
MAL02_RS02875 (MAL02_02875)	731909..733783	+	1875	WP_004425742.1	ATP-dependent DNA helicase RecQ	-
MAL02_RS02880 (MAL02_02880)	735431..735772	-	342	WP_002155521.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAL02_RS02885 (MAL02_02885)	735759..736007	-	249	WP_004425715.1	ribbon-helix-helix domain-containing protein	Antitoxin
MAL02_RS02890 (MAL02_02890)	736373..737041	-	669	Protein_571	DUF4238 domain-containing protein	-
MAL02_RS02895 (MAL02_02895)	737066..737539	-	474	WP_243823874.1	IS5 family transposase	-
MAL02_RS02900 (MAL02_02900)	737530..737844	-	315	WP_152926911.1	transposase	-
MAL02_RS02905 (MAL02_02905)	737864..738076	-	213	WP_053523145.1	hypothetical protein	-
MAL02_RS02910 (MAL02_02910)	738720..739565	-	846	WP_243820437.1	hypothetical protein	-
MAL02_RS02915 (MAL02_02915)	739567..740847	-	1281	WP_004437627.1	phosphoribosylamine--glycine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12329.22 Da
• Isoelectric Point: 9.8437

>T235274 WP_002155521.1 NZ_CP091928:c735772-735431 [Leptospira noguchii]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSSISTVVSIAITSNLNLSEAPGNVLISKKESSLSKDSVVNVSQIV
TLDKERFIKRAGKLKSSKINEVETGLKLVTGLN

Nucleotide

• Length: 342 bp

>T235274 NZ_LR134235:c2238539-2237622 [Klebsiella variicola]
ATGGATATACAGGCGCCCTATTATCGGCAGGTTGCTCTGTTAATGCAGGTTTTACCCTATGTTGCCGTCGAACGGGAATT
TGCTCTGAAAGGTGGCACGGCAATCAATCTCTTTATCCGTGATTTCCCACGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAACGATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCTAATCTGCAACAACAG
GCAGGTATGAACGCTGTATTACAAGCTAATCGTTCCGATGAGATGCGTGTCGTTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTCTCACCGGTCGCACGGGGAACATTATATCCACCACAGGAGCGAGAGGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCGGTGGTTTCGTTGCCCGATCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGATGTGAAATTACTTCTTGATGCGCAGGAGCTTAACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTTAGCCATAATCGGCCTCTGGCTGAAGTGCTTAGTCCTCGCTGGAAAGATATTGCTGAACCCTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCTACAGGATGATTGATGCGTTAAAATCC
TGTTTCACGCAACAGGATGTCGATTTTTTGCTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAACGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGTTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9337.83 Da
• Isoelectric Point: 10.3019

>AT235274 WP_004425715.1 NZ_CP091928:c736007-735759 [Leptospira noguchii]
MKTAISIPDELFKTAEKIAKKLGIPRSQLFAKALEEFIQSHSKESITEKLNKVYSNKSKELKSNIVDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT235274 NZ_LR134235:c2239303-2238545 [Klebsiella variicola]
ATGCCATCAAAATTAAACTGGCTGCTGCAAAACAGTGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCGGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACACGCAAAGTGGCTGGTTAAAAAAACGAGGCAACGGCGTATACGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTAGCCGCTCCGGTGTATGTCGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGTCGTTCACACTATCTACAGCTCAAACAAAACCAGTGCTGGTTGAGTATGGAAAATAAAGCGCT
GCTGCCAAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGCAAAAACTGCCGGTGCTGGATGATA
AATACCGCGTAATGCTTGATGTAAAAGGGAAAAGACTCACCGGCAGTGCGCCGGAACTGGCTGCTTACGAGTTGTTGAGC
GCGGTACCGGGGACCCTCTCATTTAACCACGCGGCAGAGCTTTTCCAGGGACTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTGCTTTCTGTCAGCCAGTCCGTGCAGACCAAGCGTTTGTATCTCTTTTTCGCCAGTTTTTACGAGCATGGTTGGC
TCAGACGAATCGATAGCCAAAAAATCGATCTGGGTGCGGGTAAGCGGCAAATCGTGGTAAATGGCAAGTTCAATGCTCAA
TATCAAATCACTGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: M6U786