Detailed information of TA system TA235273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 52019..52554
• Replicon: plasmid pJNQH497-2
• Accession: NZ_CP091927
• Organism: Escherichia coli strain JNQH497

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KIF99_RS24905
• Protein ID: WP_032206513.1
• Coordinates: 52267..52554 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: KIF99_RS24900
• Protein ID: WP_001139206.1
• Coordinates: 52019..52270 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIF99_RS24895 (KIF99_24895)	47469..51359	-	3891	WP_240225451.1	helicase-related protein	-
KIF99_RS24900 (KIF99_24900)	52019..52270	+	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KIF99_RS24905 (KIF99_24905)	52267..52554	+	288	WP_032206513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIF99_RS24910 (KIF99_24910)	52922..53035	-	114	Protein_62	alkaline phosphatase	-
KIF99_RS24915 (KIF99_24915)	53034..53239	+	206	Protein_63	hypothetical protein	-
KIF99_RS24920 (KIF99_24920)	53414..56929	-	3516	WP_072752622.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / oqxA / oqxB / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..101321	101321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.20 Da
• Isoelectric Point: 10.5833

>T235273 WP_032206513.1 NZ_CP091927:52267-52554 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQIIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T235273 NZ_LR134235:1338371-1338589 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT235273 WP_001139206.1 NZ_CP091927:52019-52270 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT235273 NZ_LR134235:1337970-1338344 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: T1PXV2