Detailed information of TA system TA235272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 35989..36590
• Replicon: plasmid pJNQH497-2
• Accession: NZ_CP091927
• Organism: Escherichia coli strain JNQH497

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: KIF99_RS24810
• Protein ID: WP_001216045.1
• Coordinates: 36210..36590 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KIF99_RS24805
• Protein ID: WP_001190712.1
• Coordinates: 35989..36210 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIF99_RS24775 (KIF99_24775)	31460..31732	+	273	WP_050489281.1	PerC family transcriptional regulator	-
KIF99_RS24785 (KIF99_24785)	32899..33297	+	399	Protein_37	hypothetical protein	-
KIF99_RS24790 (KIF99_24790)	33352..34049	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-
KIF99_RS24795 (KIF99_24795)	34065..34238	-	174	Protein_39	restriction endonuclease subunit S	-
KIF99_RS24800 (KIF99_24800)	34250..35806	-	1557	WP_072647227.1	type I restriction-modification system subunit M	-
KIF99_RS24805 (KIF99_24805)	35989..36210	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KIF99_RS24810 (KIF99_24810)	36210..36590	+	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KIF99_RS24815 (KIF99_24815)	36595..36774	+	180	WP_000113018.1	hypothetical protein	-
KIF99_RS24820 (KIF99_24820)	36802..37845	+	1044	WP_072647226.1	DUF968 domain-containing protein	-
KIF99_RS24825 (KIF99_24825)	37969..38937	-	969	WP_000654811.1	IS5 family transposase	-
KIF99_RS24830 (KIF99_24830)	38963..39061	+	99	Protein_46	phage antirepressor Ant	-
KIF99_RS24835 (KIF99_24835)	39094..39945	-	852	WP_000611664.1	phage repressor protein C1	-
KIF99_RS24840 (KIF99_24840)	40056..40265	-	210	WP_000874156.1	hypothetical protein	-
KIF99_RS24845 (KIF99_24845)	40436..41133	+	698	WP_103215986.1	IS1-like element IS1A family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1 / oqxA / oqxB / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..101321	101321
	-	inside	IScluster/Tn	-	-	28613..41133	12520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T235272 WP_001216045.1 NZ_CP091927:36210-36590 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T235272 NZ_LR134235:128273-128425 [Klebsiella variicola]
ATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTGCTGATATACCC
GAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAAGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT235272 WP_001190712.1 NZ_CP091927:35989-36210 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT235272 NZ_LR134235:128474-128776 [Klebsiella variicola]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTGATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6