Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 89835..91458 Replicon plasmid pJNQH497-1
Accession NZ_CP091926
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KIF99_RS23640 Protein ID WP_000088046.1
Coordinates 89835..91160 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KIF99_RS23645 Protein ID WP_024136327.1
Coordinates 91165..91458 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS23605 (KIF99_23605) 85554..85757 - 204 WP_001194555.1 hypothetical protein -
KIF99_RS23610 (KIF99_23610) 85819..86676 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KIF99_RS23615 (KIF99_23615) 86692..87000 - 309 WP_001224687.1 hypothetical protein -
KIF99_RS23620 (KIF99_23620) 87549..87974 - 426 WP_001043843.1 hypothetical protein -
KIF99_RS23625 (KIF99_23625) 88228..89043 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
KIF99_RS23630 (KIF99_23630) 89056..89466 - 411 WP_000985911.1 hypothetical protein -
KIF99_RS23635 (KIF99_23635) 89568..89774 - 207 WP_000134171.1 hypothetical protein -
KIF99_RS23640 (KIF99_23640) 89835..91160 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KIF99_RS23645 (KIF99_23645) 91165..91458 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KIF99_RS23650 (KIF99_23650) 92062..92454 - 393 WP_000341066.1 hypothetical protein -
KIF99_RS23655 (KIF99_23655) 92827..93168 + 342 WP_001151575.1 hypothetical protein -
KIF99_RS23660 (KIF99_23660) 93387..96404 + 3018 WP_004199413.1 Tn3-like element IS3000 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / mph(A) / erm(42) / sul2 / aph(4)-Ia / aac(3)-IVa / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / ant(3'')-Ia / tet(M) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..263021 263021
- flank IS/Tn - - 82379..85396 3017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T235271 WP_000088046.1 NZ_CP091926:c91160-89835 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T235271 NZ_LR134235:72186-72497 [Klebsiella variicola]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCAAGACACTATCCGAATGATGCAGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAGAAATGGTGGGTTATCGATATCGGCGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT235271 WP_024136327.1 NZ_CP091926:c91458-91165 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT235271 NZ_LR134235:72494-72910 [Klebsiella variicola]
ATGATTTTTAGCGATGCCATTAAAGCTGCGAATGACCTGGCCAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATACCTGGGAAGATAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTCGAAAATGAGATAGGTAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References