Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4109923..4110499 Replicon chromosome
Accession NZ_CP091925
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name shpA Uniprot ID A0A2X2XYK1
Locus tag KIF99_RS20190 Protein ID WP_001360091.1
Coordinates 4110212..4110499 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KIF99_RS20185 Protein ID WP_000063156.1
Coordinates 4109923..4110225 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS20170 (4106519) 4106519..4108669 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KIF99_RS20175 (4108719) 4108719..4108922 + 204 WP_000467859.1 YbdD/YjiX family protein -
KIF99_RS20180 (4108933) 4108933..4109889 + 957 WP_001297640.1 GTPase -
KIF99_RS20185 (4109923) 4109923..4110225 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KIF99_RS20190 (4110212) 4110212..4110499 - 288 WP_001360091.1 BrnT family toxin Toxin
KIF99_RS20195 (4110698) 4110698..4111395 - 698 Protein_3967 IS1-like element IS1F family transposase -
KIF99_RS20200 (4111452) 4111452..4111547 + 96 Protein_3968 dienelactone hydrolase family protein -
KIF99_RS20205 (4111693) 4111693..4112925 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
KIF99_RS20210 (4112966) 4112966..4114246 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11325.80 Da        Isoelectric Point: 6.8821

>T235269 WP_001360091.1 NZ_CP091925:c4110499-4110212 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKTDRKERNRYEHG

Download         Length: 288 bp

>T235269 NZ_LR134234:c4426326-4425784 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT235269 WP_000063156.1 NZ_CP091925:c4110225-4109923 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT235269 NZ_LR134234:c4426621-4426310 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X2XYK1

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References