Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3485031..3485649 Replicon chromosome
Accession NZ_CP091925
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KIF99_RS17050 Protein ID WP_001291435.1
Coordinates 3485431..3485649 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KIF99_RS17045 Protein ID WP_000344800.1
Coordinates 3485031..3485405 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS17035 (3480120) 3480120..3481313 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KIF99_RS17040 (3481336) 3481336..3484485 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KIF99_RS17045 (3485031) 3485031..3485405 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KIF99_RS17050 (3485431) 3485431..3485649 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KIF99_RS17055 (3485821) 3485821..3486372 + 552 WP_000102564.1 maltose O-acetyltransferase -
KIF99_RS17060 (3486488) 3486488..3486958 + 471 WP_000136192.1 YlaC family protein -
KIF99_RS17065 (3487122) 3487122..3488672 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KIF99_RS17070 (3488714) 3488714..3489067 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KIF99_RS17080 (3489446) 3489446..3489757 + 312 WP_000409911.1 MGMT family protein -
KIF99_RS17085 (3489788) 3489788..3490360 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T235265 WP_001291435.1 NZ_CP091925:3485431-3485649 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T235265 NZ_LR134234:3385685-3385996 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT235265 WP_000344800.1 NZ_CP091925:3485031-3485405 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT235265 NZ_LR134234:3385997-3386287 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References