Detailed information of TA system TA235264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2419862..2420500
• Replicon: chromosome
• Accession: NZ_CP091925
• Organism: Escherichia coli strain JNQH497

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KIF99_RS11820
• Protein ID: WP_000813794.1
• Coordinates: 2420324..2420500 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KIF99_RS11815
• Protein ID: WP_001270286.1
• Coordinates: 2419862..2420278 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIF99_RS11795 (2415014)	2415014..2415955	-	942	WP_001251304.1	ABC transporter permease	-
KIF99_RS11800 (2415956)	2415956..2416969	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KIF99_RS11805 (2416987)	2416987..2418132	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KIF99_RS11810 (2418377)	2418377..2419783	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KIF99_RS11815 (2419862)	2419862..2420278	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KIF99_RS11820 (2420324)	2420324..2420500	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KIF99_RS11825 (2420722)	2420722..2420952	+	231	WP_000494244.1	YncJ family protein	-
KIF99_RS11830 (2421044)	2421044..2423005	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KIF99_RS11835 (2423078)	2423078..2423614	-	537	WP_080317363.1	DNA-binding transcriptional regulator SutR	-
KIF99_RS11840 (2423706)	2423706..2424881	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T235264 WP_000813794.1 NZ_CP091925:c2420500-2420324 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T235264 NZ_LR134234:c2882861-2882334 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT235264 WP_001270286.1 NZ_CP091925:c2420278-2419862 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT235264 NZ_LR134234:c2883134-2882868 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 6HPB