Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 841757..842411 Replicon chromosome
Accession NZ_CP091925
Organism Escherichia coli strain JNQH497

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KIF99_RS04105 Protein ID WP_000244777.1
Coordinates 842004..842411 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KIF99_RS04100 Protein ID WP_000354046.1
Coordinates 841757..842023 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIF99_RS04075 (836926) 836926..837669 + 744 WP_000951964.1 SDR family oxidoreductase -
KIF99_RS04080 (837726) 837726..839159 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KIF99_RS04085 (839204) 839204..839515 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KIF99_RS04090 (839679) 839679..840338 + 660 WP_000250274.1 hemolysin III family protein -
KIF99_RS04095 (840534) 840534..841514 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KIF99_RS04100 (841757) 841757..842023 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KIF99_RS04105 (842004) 842004..842411 + 408 WP_000244777.1 protein YgfX Toxin
KIF99_RS04110 (842451) 842451..842972 - 522 WP_039020580.1 flavodoxin FldB -
KIF99_RS04115 (843084) 843084..843980 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KIF99_RS04120 (844005) 844005..844715 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KIF99_RS04125 (844721) 844721..846454 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T235250 WP_000244777.1 NZ_CP091925:842004-842411 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T235250 NZ_LR134231:c3574892-3574494 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT235250 WP_000354046.1 NZ_CP091925:841757-842023 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT235250 NZ_LR134231:c3575188-3574895 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References