Detailed information of TA system TA023525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 109395..109927
• Replicon: plasmid pAtS4e
• Accession: NC_011981
• Organism: Agrobacterium vitis S4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: B9K4Q0
• Locus tag: AVI_RS26235
• Protein ID: WP_015918290.1
• Coordinates: 109395..109688 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: AVI_RS26240
• Protein ID: WP_041699959.1
• Coordinates: 109685..109927 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVI_RS26215	105491..106360	+	870	WP_015918286.1	ABC transporter permease	-
AVI_RS26220	106364..107209	+	846	WP_015918287.1	ABC transporter permease subunit	-
AVI_RS26225	107237..108310	+	1074	WP_015918288.1	ABC transporter ATP-binding protein	-
AVI_RS26230	108360..109218	-	859	Protein_102	IS481 family transposase	-
AVI_RS26235	109395..109688	-	294	WP_015918290.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AVI_RS26240	109685..109927	-	243	WP_041699959.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
AVI_RS30825	110221..110400	-	180	Protein_105	IS6 family transposase	-
AVI_RS26250	110555..111057	+	503	Protein_106	AraC family transcriptional regulator	-
AVI_RS30830	111346..111663	+	318	WP_049777523.1	nuclear transport factor 2 family protein	-
AVI_RS26260	112177..113175	-	999	WP_015918294.1	DUF4892 domain-containing protein	-
AVI_RS26265	113698..114129	+	432	WP_041699702.1	MucR family transcriptional regulator	-
AVI_RS26270	114361..114690	+	330	WP_015918295.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..631775	631775
	-	flank	IS/Tn	-	-	108406..109317	911
	-	flank	IS/Tn	-	-	114792..115490	698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11347.99 Da
• Isoelectric Point: 5.5613

>T23525 WP_015918290.1 NC_011981:c109688-109395 [Agrobacterium vitis S4]
MSFKLSVEAEEDIITIAEQGARMFGVDQAKRYHDELFALFDLIAANPRIARERNEIEPAVRIHPFKAHLIVYRVEDDEKI
FVVRIRHGHEDWVSISM

Nucleotide

• Length: 294 bp

>T23525 NC_011981:c109688-109395 [Agrobacterium vitis S4]
ATGAGTTTTAAACTTTCTGTCGAGGCAGAAGAAGACATCATTACGATCGCCGAGCAGGGTGCCCGTATGTTCGGAGTAGA
CCAGGCAAAACGCTACCATGATGAACTTTTCGCACTATTTGATCTGATCGCTGCTAATCCACGCATCGCACGCGAACGGA
ATGAGATCGAACCGGCCGTCAGAATTCATCCCTTCAAAGCACATCTTATCGTCTATCGGGTTGAAGACGATGAGAAAATT
TTCGTAGTGCGAATTCGCCACGGGCATGAAGATTGGGTAAGTATTTCAATGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8926.02 Da
• Isoelectric Point: 6.2961

>AT23525 WP_041699959.1 NC_011981:c109927-109685 [Agrobacterium vitis S4]
MATMNVSLPDPMKDWVEAQARTGRYSNASDYVRDLIRRDQTRNDKIAAMQGFVEAGLQSGVGIRSKDELFAEAMDRASKS

Nucleotide

• Length: 243 bp

>AT23525 NC_011981:c109927-109685 [Agrobacterium vitis S4]
ATGGCAACAATGAACGTATCTCTTCCAGACCCGATGAAGGATTGGGTGGAGGCGCAGGCCAGGACTGGGCGATATTCGAA
CGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACACGAAACGACAAGATTGCCGCCATGCAAGGTTTCGTCG
AGGCCGGGCTCCAAAGTGGCGTTGGCATTCGATCAAAGGATGAACTCTTTGCTGAGGCGATGGATCGTGCCAGCAAGTCA
TGA

Structures

Toxin

• AlphaFold DB: B9K4Q0