Detailed information of TA system TA235232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 36406..36970
• Replicon: plasmid p1
• Accession: NZ_CP091907
• Organism: Enterococcus faecalis strain 2-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: A0A0M2A908
• Locus tag: L5I26_RS13355
• Protein ID: WP_010724880.1
• Coordinates: 36680..36970 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: L5I26_RS13350
• Protein ID: WP_002331065.1
• Coordinates: 36406..36678 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I26_RS13315 (31642)	31642..32322	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
L5I26_RS13320 (32377)	32377..32613	+	237	Protein_32	aminoglycoside 6-adenylyltransferase	-
L5I26_RS13325 (32610)	32610..33152	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
L5I26_RS13330 (33245)	33245..34039	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
L5I26_RS13335 (34315)	34315..34887	+	573	WP_001079938.1	HTH domain-containing protein	-
L5I26_RS13340 (35185)	35185..36081	+	897	WP_002334978.1	AAA family ATPase	-
L5I26_RS13345 (36180)	36180..36389	+	210	WP_000527318.1	peptide-binding protein	-
L5I26_RS13350 (36406)	36406..36678	+	273	WP_002331065.1	antitoxin	Antitoxin
L5I26_RS13355 (36680)	36680..36970	+	291	WP_010724880.1	zeta toxin family protein	Toxin
L5I26_RS13360 (36998)	36998..37678	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
L5I26_RS13365 (37720)	37720..38034	+	315	WP_238461345.1	hypothetical protein	-
L5I26_RS13370 (38195)	38195..39784	+	1590	WP_142425799.1	peptide ABC transporter substrate-binding protein	-
L5I26_RS13375 (39811)	39811..40803	-	993	WP_226094930.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / erm(B)	cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / EF0149 / asa1	1..149744	149744
	-	inside	IScluster/Tn	aph(3')-III	-	30868..37678	6810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-81)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10902.37 Da
• Isoelectric Point: 5.8599

>T235232 WP_010724880.1 NZ_CP091907:36680-36970 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KRFCCKVLNLLSNKVE

Nucleotide

• Length: 291 bp

>T235232 NZ_LR134228:c3854803-3854405 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT235232 WP_002331065.1 NZ_CP091907:36406-36678 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT235232 NZ_LR134228:c3855099-3854806 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0M2A908

Antitoxin

• AlphaFold DB: A0A828ZL55