Detailed information of TA system TA023523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-shpB/BrnT-BrnA
• Location: 3085103..3085640
• Replicon: chromosome
• Accession: NC_011979
• Organism: Geobacter daltonii FRC-32

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: B9M250
• Locus tag: GEOB_RS13980
• Protein ID: WP_012647896.1
• Coordinates: 3085356..3085640 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B9M249
• Locus tag: GEOB_RS13975
• Protein ID: WP_012647895.1
• Coordinates: 3085103..3085372 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEOB_RS13945	3081029..3081802	-	774	WP_012647891.1	hypothetical protein	-
GEOB_RS13950	3081763..3082176	-	414	WP_085950222.1	L,D-transpeptidase	-
GEOB_RS13955	3082592..3082813	-	222	Protein_2774	hypothetical protein	-
GEOB_RS13960	3082895..3083644	-	750	WP_012647892.1	hypothetical protein	-
GEOB_RS13965	3083814..3084338	-	525	WP_012647893.1	L,D-transpeptidase family protein	-
GEOB_RS13970	3084675..3084968	-	294	WP_012647894.1	nucleotidyltransferase domain-containing protein	-
GEOB_RS13975	3085103..3085372	-	270	WP_012647895.1	BrnA antitoxin family protein	Antitoxin
GEOB_RS13980	3085356..3085640	-	285	WP_012647896.1	BrnT family toxin	Toxin
GEOB_RS13985	3085701..3086348	-	648	WP_041267155.1	RES family NAD+ phosphorylase	-
GEOB_RS13990	3086380..3086766	-	387	WP_012647898.1	XRE family transcriptional regulator	-
GEOB_RS20175	3086867..3087025	+	159	WP_154650499.1	hypothetical protein	-
GEOB_RS14000	3087441..3088046	-	606	WP_012647899.1	PEP-CTERM sorting domain-containing protein	-
GEOB_RS19825	3088375..3088548	-	174	WP_012647900.1	hypothetical protein	-
GEOB_RS14005	3088545..3089276	-	732	WP_012647901.1	HD-GYP domain-containing protein	-
GEOB_RS14010	3089711..3090121	-	411	WP_012647902.1	(deoxy)nucleoside triphosphate pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-86)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-81)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10965.40 Da
• Isoelectric Point: 5.6618

>T23523 WP_012647896.1 NC_011979:c3085640-3085356 [Geobacter daltonii FRC-32]
VQLVWDENKNTSNKIKHKVSFELASLVFDDPFHLSVLDRIENGEERWQTLGLVGNVVVLLVAHSFVEQDDDEMIRIISAR
KATKKERQRYEEGR

Nucleotide

• Length: 285 bp

>T23523 NC_011979:c3085640-3085356 [Geobacter daltonii FRC-32]
GTGCAGCTTGTCTGGGACGAAAACAAAAATACCAGCAACAAGATTAAGCACAAGGTAAGTTTTGAACTAGCCAGCCTTGT
TTTCGATGACCCTTTTCACCTTAGCGTTCTCGACCGGATCGAAAACGGAGAGGAACGCTGGCAGACGTTGGGCTTGGTGG
GCAACGTGGTCGTGCTGCTGGTGGCTCATTCCTTCGTCGAGCAGGATGATGACGAGATGATCCGCATCATTTCCGCCCGC
AAGGCAACGAAGAAAGAGAGGCAACGATATGAAGAAGGCAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10127.57 Da
• Isoelectric Point: 8.5277

>AT23523 WP_012647895.1 NC_011979:c3085372-3085103 [Geobacter daltonii FRC-32]
MKKADKKLADELAVLAAISDEEIDTSEIPENTDWSSAVVGRFYRPVKEIVTIRLDADVLDWLKQGGKGYQTRINRILRSA
MEHQRKRAA

Nucleotide

• Length: 270 bp

>AT23523 NC_011979:c3085372-3085103 [Geobacter daltonii FRC-32]
ATGAAGAAGGCAGATAAAAAGTTGGCTGATGAATTGGCGGTCCTTGCCGCAATATCCGATGAAGAGATTGACACTTCCGA
GATTCCGGAGAACACTGACTGGAGCAGCGCCGTCGTCGGGCGTTTTTACCGGCCGGTAAAAGAGATCGTTACTATTCGTC
TGGATGCCGACGTGCTTGACTGGCTGAAGCAGGGGGGAAAGGGCTATCAGACCAGGATCAACAGGATTCTTAGGTCTGCT
ATGGAGCACCAGCGGAAGAGGGCAGCCTGA

Structures

Toxin

• AlphaFold DB: B9M250

Antitoxin

• AlphaFold DB: B9M249