235220

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2638657..2639228 Replicon chromosome
Accession NZ_CP091906
Organism Enterococcus faecalis strain 2-1

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag L5I26_RS12635 Protein ID WP_002360937.1
Coordinates 2638657..2638998 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag L5I26_RS12640 Protein ID WP_002367500.1
Coordinates 2638998..2639228 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I26_RS12630 (2634672) 2634672..2638286 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
L5I26_RS12635 (2638657) 2638657..2638998 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5I26_RS12640 (2638998) 2638998..2639228 - 231 WP_002367500.1 hypothetical protein Antitoxin
L5I26_RS12645 (2639634) 2639634..2639849 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
L5I26_RS12650 (2639988) 2639988..2640980 + 993 WP_002398510.1 biotin--[acetyl-CoA-carboxylase] ligase -
L5I26_RS12655 (2641147) 2641147..2641785 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
L5I26_RS12660 (2642469) 2642469..2644085 + 1617 WP_002372620.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T235220 WP_002360937.1 NZ_CP091906:c2638998-2638657 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T235220 NZ_LR134228:1386885-1387283 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT235220 WP_002367500.1 NZ_CP091906:c2639228-2638998 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT235220 NZ_LR134228:1386658-1386885 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References