Detailed information of TA system TA023521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 2457059..2457687
• Replicon: chromosome
• Accession: NC_011979
• Organism: Geobacter daltonii FRC-32

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B9M9K0
• Locus tag: GEOB_RS11035
• Protein ID: WP_012647301.1
• Coordinates: 2457059..2457460 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B9M9K1
• Locus tag: GEOB_RS11040
• Protein ID: WP_012647302.1
• Coordinates: 2457460..2457687 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEOB_RS11000	2452287..2452649	+	363	WP_012647293.1	CidA/LrgA family protein	-
GEOB_RS11005	2452646..2453365	+	720	WP_012647294.1	LrgB family protein	-
GEOB_RS11010	2453402..2453911	+	510	WP_012647295.1	shikimate kinase	-
GEOB_RS11015	2453988..2454329	+	342	WP_012647296.1	metalloregulator ArsR/SmtB family transcription factor	-
GEOB_RS11020	2454392..2455435	+	1044	WP_012647297.1	ACR3 family arsenite efflux transporter	-
GEOB_RS11025	2455656..2456336	+	681	WP_012647298.1	DUF2461 domain-containing protein	-
GEOB_RS11030	2456461..2457012	+	552	WP_012647300.1	hypothetical protein	-
GEOB_RS11035	2457059..2457460	-	402	WP_012647301.1	type II toxin-antitoxin system VapC family toxin	Toxin
GEOB_RS11040	2457460..2457687	-	228	WP_012647302.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GEOB_RS11045	2457817..2458188	-	372	WP_012647303.1	pyridoxamine 5'-phosphate oxidase family protein	-
GEOB_RS11050	2458580..2459239	+	660	WP_012647304.1	hypothetical protein	-
GEOB_RS11055	2459285..2459983	+	699	WP_012647305.1	hypothetical protein	-
GEOB_RS11060	2459992..2461296	+	1305	WP_154650565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14724.07 Da
• Isoelectric Point: 5.1546

>T23521 WP_012647301.1 NC_011979:c2457460-2457059 [Geobacter daltonii FRC-32]
MKLLLDTNICIYIIKQQPDTVLKHFLEYEIGDIGISSITLSELRYGVAKSAHREKNAKALDEFIIPLEVVAYDEAAAHVY
GDIRSTLEKAGSPIGSMDMLIAAHAVSLGIPLVTNNTREFLRVPSINLLDWTV

Nucleotide

• Length: 402 bp

>T23521 NC_011979:c2457460-2457059 [Geobacter daltonii FRC-32]
ATGAAACTGCTGCTCGACACCAATATCTGTATTTATATCATCAAGCAACAGCCGGACACCGTGTTGAAGCATTTTCTGGA
GTACGAGATCGGTGACATTGGCATTTCCTCCATAACCCTCTCCGAACTGCGCTACGGGGTCGCAAAAAGCGCTCACCGAG
AAAAAAACGCCAAAGCTCTCGACGAGTTCATCATCCCCCTGGAGGTGGTCGCTTATGATGAAGCGGCTGCCCACGTTTAT
GGAGATATCAGATCGACTCTTGAAAAAGCCGGCTCCCCCATTGGTTCCATGGATATGCTCATTGCCGCTCACGCCGTATC
ATTGGGAATCCCTCTGGTTACAAATAACACCCGCGAGTTTTTGCGCGTCCCCTCCATCAACCTTCTTGATTGGACAGTTT
GA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8806.00 Da
• Isoelectric Point: 9.1844

>AT23521 WP_012647302.1 NC_011979:c2457687-2457460 [Geobacter daltonii FRC-32]
MKTAKIFQNGQSQAVRLPKEFRFDDSEVFIKKCGNIVQLIPRSDSWDSLFGSLKKFSSDFMSERIQPEQDKRENF

Nucleotide

• Length: 228 bp

>AT23521 NC_011979:c2457687-2457460 [Geobacter daltonii FRC-32]
ATGAAAACAGCAAAAATATTTCAAAATGGACAAAGCCAGGCAGTCAGGCTCCCTAAGGAATTTCGCTTCGATGACAGCGA
GGTATTCATAAAGAAATGCGGCAACATCGTCCAATTGATCCCCCGGAGCGATTCGTGGGATTCTTTATTCGGCAGTCTCA
AAAAATTCTCAAGCGACTTCATGTCTGAACGCATACAACCTGAACAGGACAAGCGGGAAAATTTCTGA

Structures

Toxin

• AlphaFold DB: B9M9K0

Antitoxin

• AlphaFold DB: B9M9K1