235200

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 45059..45623 Replicon plasmid p1
Accession NZ_CP091905
Organism Enterococcus faecalis strain 43-2

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2A908
Locus tag L5I20_RS15075 Protein ID WP_010724880.1
Coordinates 45333..45623 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag L5I20_RS15070 Protein ID WP_002331065.1
Coordinates 45059..45331 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I20_RS15040 (40358) 40358..41266 + 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
L5I20_RS15045 (41263) 41263..41805 + 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
L5I20_RS15050 (41898) 41898..42692 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
L5I20_RS15055 (42968) 42968..43540 + 573 WP_001079938.1 HTH domain-containing protein -
L5I20_RS15060 (43838) 43838..44734 + 897 WP_002334978.1 AAA family ATPase -
L5I20_RS15065 (44833) 44833..45042 + 210 WP_000527318.1 peptide-binding protein -
L5I20_RS15070 (45059) 45059..45331 + 273 WP_002331065.1 antitoxin Antitoxin
L5I20_RS15075 (45333) 45333..45623 + 291 WP_010724880.1 zeta toxin family protein Toxin
L5I20_RS15080 (45651) 45651..46331 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
L5I20_RS15085 (46379) 46379..46453 - 75 Protein_50 rRNA adenine methyltransferase -
L5I20_RS15090 (46458) 46458..47195 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
L5I20_RS15095 (47320) 47320..47403 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
L5I20_RS15100 (47526) 47526..47693 - 168 Protein_53 peptide-binding protein -
L5I20_RS15105 (47827) 47827..48087 - 261 Protein_54 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
L5I20_RS15110 (48267) 48267..48947 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
L5I20_RS15115 (48989) 48989..49279 + 291 WP_238461556.1 hypothetical protein -
L5I20_RS15120 (49273) 49273..49497 + 225 WP_002406593.1 ultraviolet resistance protein UvrA repressor UvrC -
L5I20_RS15125 (49558) 49558..49767 + 210 WP_010822384.1 hypothetical protein -
L5I20_RS15130 (49779) 49779..50081 + 303 WP_002406176.1 DUF6440 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) cylR2 / cylL / cylS / cylM / cylB / cylA / cylI 1..54858 54858
- inside IScluster/Tn ant(6)-Ia / aph(3')-III / erm(B) - 30868..48947 18079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-81)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10902.37 Da        Isoelectric Point: 5.8599

>T235200 WP_010724880.1 NZ_CP091905:45333-45623 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KRFCCKVLNLLSNKVE

Download         Length: 291 bp

>T235200 NZ_LR134227:2233915-2234202 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT235200 WP_002331065.1 NZ_CP091905:45059-45331 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp

>AT235200 NZ_LR134227:2233676-2233915 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A908

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References