Detailed information of TA system TA235195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2892833..2893404
• Replicon: chromosome
• Accession: NZ_CP091904
• Organism: Enterococcus faecalis strain 43-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5I20_RS14380
• Protein ID: WP_002385138.1
• Coordinates: 2892833..2893174 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: L5I20_RS14385
• Protein ID: WP_002354773.1
• Coordinates: 2893174..2893404 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I20_RS14375 (2888848)	2888848..2892462	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
L5I20_RS14380 (2892833)	2892833..2893174	-	342	WP_002385138.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I20_RS14385 (2893174)	2893174..2893404	-	231	WP_002354773.1	hypothetical protein	Antitoxin
L5I20_RS14390 (2893810)	2893810..2894025	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
L5I20_RS14395 (2894164)	2894164..2895156	+	993	WP_002385136.1	biotin--[acetyl-CoA-carboxylase] ligase	-
L5I20_RS14400 (2895420)	2895420..2896058	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
L5I20_RS14405 (2896746)	2896746..2898362	+	1617	WP_002372620.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13128.35 Da
• Isoelectric Point: 9.3984

>T235195 WP_002385138.1 NZ_CP091904:c2893174-2892833 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAAICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T235195 NZ_LR134227:836357-836764 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT235195 WP_002354773.1 NZ_CP091904:c2893404-2893174 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT235195 NZ_LR134227:836110-836376 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812