235180

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 79324..80461 Replicon plasmid p1
Accession NZ_CP091902
Organism Enterococcus faecalis strain 59

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag L5I17_RS15430 Protein ID WP_001284311.1
Coordinates 79598..80461 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag L5I17_RS15425 Protein ID WP_000301765.1
Coordinates 79324..79596 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I17_RS15400 (75056) 75056..75226 + 171 WP_000713594.1 hypothetical protein -
L5I17_RS15405 (75240) 75240..75857 + 618 WP_001062589.1 recombinase family protein -
L5I17_RS15410 (75857) 75857..78001 + 2145 WP_000108741.1 type IA DNA topoisomerase -
L5I17_RS15415 (78104) 78104..79000 + 897 WP_001835294.1 ParA family protein -
L5I17_RS15420 (79092) 79092..79307 + 216 WP_001835296.1 peptide-binding protein -
L5I17_RS15425 (79324) 79324..79596 + 273 WP_000301765.1 antitoxin Antitoxin
L5I17_RS15430 (79598) 79598..80461 + 864 WP_001284311.1 zeta toxin family protein Toxin
L5I17_RS15435 (80650) 80650..81228 + 579 WP_238464146.1 TetR/AcrR family transcriptional regulator -
L5I17_RS15440 (81260) 81260..81703 + 444 WP_032490933.1 small multi-drug export protein -
L5I17_RS15445 (81856) 81856..82227 + 372 WP_238464148.1 transposase -
L5I17_RS15450 (82255) 82255..82596 - 342 WP_238464150.1 hypothetical protein -
L5I17_RS15455 (82614) 82614..82814 - 201 WP_238464151.1 hypothetical protein -
L5I17_RS15460 (83341) 83341..84078 - 738 WP_128888465.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
L5I17_RS15465 (84203) 84203..84286 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / erm(B) - 1..88965 88965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T235180 WP_001284311.1 NZ_CP091902:79598-80461 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T235180 NZ_CP091902:79598-80461 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT235180 WP_000301765.1 NZ_CP091902:79324-79596 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT235180 NZ_CP091902:79324-79596 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References