235179

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 70538..71109 Replicon plasmid p1
Accession NZ_CP091902
Organism Enterococcus faecalis strain 59

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag L5I17_RS15370 Protein ID WP_001278770.1
Coordinates 70538..70879 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag L5I17_RS15375 Protein ID WP_000958277.1
Coordinates 70879..71109 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I17_RS15345 (66207) 66207..66533 + 327 WP_000606299.1 hypothetical protein -
L5I17_RS15350 (67392) 67392..67667 - 276 WP_000889655.1 type II toxin-antitoxin system YafQ family toxin -
L5I17_RS15355 (67660) 67660..67926 - 267 WP_000246197.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
L5I17_RS15360 (68639) 68639..69241 - 603 WP_000892301.1 Fic family protein -
L5I17_RS15365 (69488) 69488..70426 - 939 WP_001053446.1 hypothetical protein -
L5I17_RS15370 (70538) 70538..70879 - 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5I17_RS15375 (70879) 70879..71109 - 231 WP_000958277.1 hypothetical protein Antitoxin
L5I17_RS15380 (71339) 71339..71944 + 606 WP_000599739.1 Fic family protein -
L5I17_RS15385 (71960) 71960..72532 + 573 WP_000170424.1 recombinase family protein -
L5I17_RS15390 (72965) 72965..73924 - 960 WP_000222577.1 IS30-like element IS1252 family transposase -
L5I17_RS15395 (74052) 74052..74732 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
L5I17_RS15400 (75056) 75056..75226 + 171 WP_000713594.1 hypothetical protein -
L5I17_RS15405 (75240) 75240..75857 + 618 WP_001062589.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') / erm(B) - 1..88965 88965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T235179 WP_001278770.1 NZ_CP091902:c70879-70538 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T235179 NZ_LR134226:1956272-1956375 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT235179 WP_000958277.1 NZ_CP091902:c71109-70879 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT235179 NZ_LR134226:c1956413-1956142 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3HR30

Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References