Detailed information of TA system TA235178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 47495..48114
• Replicon: plasmid p1
• Accession: NZ_CP091902
• Organism: Enterococcus faecalis strain 59

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: L5I17_RS15260
• Protein ID: WP_000241511.1
• Coordinates: 47752..48114 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: L5I17_RS15255
• Protein ID: WP_000245205.1
• Coordinates: 47495..47758 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I17_RS15850 (42633)	42633..42764	+	132	WP_000159353.1	hypothetical protein	-
L5I17_RS15240 (42754)	42754..44067	+	1314	WP_002383787.1	hypothetical protein	-
L5I17_RS15245 (44449)	44449..45090	+	642	WP_000406546.1	hypothetical protein	-
L5I17_RS15250 (46341)	46341..46997	+	657	WP_000411487.1	hypothetical protein	-
L5I17_RS15255 (47495)	47495..47758	+	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
L5I17_RS15260 (47752)	47752..48114	+	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I17_RS15265 (48179)	48179..49141	-	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
L5I17_RS15270 (49143)	49143..50582	-	1440	WP_238468254.1	sucrose-6-phosphate hydrolase	-
L5I17_RS15275 (50773)	50773..52704	+	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'') / erm(B)	-	1..88965	88965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T235178 WP_000241511.1 NZ_CP091902:47752-48114 [Enterococcus faecalis]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T235178 NZ_LR134226:1779511-1779765 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT235178 WP_000245205.1 NZ_CP091902:47495-47758 [Enterococcus faecalis]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT235178 NZ_LR134226:1779263-1779514 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1