Detailed information of TA system TA235173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2991196..2991767
• Replicon: chromosome
• Accession: NZ_CP091901
• Organism: Enterococcus faecalis strain 59

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5I17_RS14540
• Protein ID: WP_002354774.1
• Coordinates: 2991196..2991537 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: L5I17_RS14545
• Protein ID: WP_002354773.1
• Coordinates: 2991537..2991767 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I17_RS14535 (2987211)	2987211..2990825	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
L5I17_RS14540 (2991196)	2991196..2991537	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I17_RS14545 (2991537)	2991537..2991767	-	231	WP_002354773.1	hypothetical protein	Antitoxin
L5I17_RS14550 (2991941)	2991941..2992156	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
L5I17_RS14555 (2992295)	2992295..2993287	+	993	WP_002354765.1	biotin--[acetyl-CoA-carboxylase] ligase	-
L5I17_RS14560 (2993551)	2993551..2994180	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
L5I17_RS14565 (2994873)	2994873..2996489	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2996598..2997890	1292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T235173 WP_002354774.1 NZ_CP091901:c2991537-2991196 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T235173 NZ_LR134226:c594141-593677 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCTTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT235173 WP_002354773.1 NZ_CP091901:c2991767-2991537 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT235173 NZ_LR134226:c594476-594141 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812