Detailed information of TA system TA023516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1232044..1232489
• Replicon: chromosome
• Accession: NC_011978
• Organism: Thermotoga neapolitana DSM 4359

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D2C4B8
• Locus tag: CTN_RS06365
• Protein ID: WP_008195297.1
• Coordinates: 1232259..1232489 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: Q9X145
• Locus tag: CTN_RS06360
• Protein ID: WP_004083352.1
• Coordinates: 1232044..1232262 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTN_RS06350	1228357..1229748	-	1392	WP_004083362.1	radical SAM protein	-
CTN_RS09815	1229850..1229945	-	96	WP_010865309.1	Cys-rich RiPP peptide	-
CTN_RS09940	1230022..1230186	-	165	WP_004083364.1	hypothetical protein	-
CTN_RS06355	1230382..1232031	+	1650	WP_015919755.1	helix-turn-helix domain-containing protein	-
CTN_RS06360	1232044..1232262	+	219	WP_004083352.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CTN_RS06365	1232259..1232489	+	231	WP_008195297.1	type II toxin-antitoxin system HicA family toxin	Toxin
CTN_RS06370	1232512..1232724	+	213	WP_004079908.1	hypothetical protein	-
CTN_RS09730	1232714..1232863	+	150	WP_081434644.1	type II toxin-antitoxin system HicA family toxin	-
CTN_RS06375	1232934..1234676	-	1743	WP_004079909.1	ABC transporter ATP-binding protein/permease	-
CTN_RS06380	1234739..1235509	-	771	WP_011943918.1	ABC transporter permease	-
CTN_RS06385	1235513..1235947	-	435	WP_004079914.1	ABC transporter permease	-
CTN_RS06390	1236165..1236971	-	807	WP_004079915.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1212840..1240840	28000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-64)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (6-57)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8881.54 Da
• Isoelectric Point: 11.0394

>T23516 WP_008195297.1 NC_011978:1232259-1232489 [Thermotoga neapolitana DSM 4359]
MSYLPIVDPKTMEKVLLKLGFQRVRQKGSHVFYRHSNGRYTTIPFHARALPKSLIRKIIREAGISVEEFKKVLENL

Nucleotide

• Length: 231 bp

>T23516 NC_011978:1232259-1232489 [Thermotoga neapolitana DSM 4359]
TTGAGTTATCTTCCAATAGTTGATCCAAAAACTATGGAAAAGGTTTTACTGAAACTTGGATTTCAACGTGTTCGCCAAAA
AGGAAGTCATGTGTTCTACAGGCACAGCAATGGCAGATATACGACCATCCCATTTCATGCAAGAGCCTTGCCCAAATCAC
TTATAAGAAAAATCATCCGTGAAGCGGGTATATCAGTGGAGGAATTCAAGAAAGTACTTGAGAATCTGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8215.35 Da
• Isoelectric Point: 4.0887

>AT23516 WP_004083352.1 NC_011978:1232044-1232262 [Thermotoga neapolitana DSM 4359]
MRKIFTAIIEYDPETKQYVGMVPDVPGVHTVGSSLEEVRRNLKEVLELVLEEAGDEINHQEFVALEMIEVET

Nucleotide

• Length: 219 bp

>AT23516 NC_011978:1232044-1232262 [Thermotoga neapolitana DSM 4359]
ATGCGTAAAATTTTCACAGCTATCATTGAATACGATCCTGAAACAAAGCAATATGTTGGAATGGTTCCGGACGTTCCGGG
TGTGCATACTGTAGGTAGTTCCTTAGAAGAAGTGAGAAGGAATTTAAAAGAGGTGCTTGAACTCGTATTGGAGGAAGCAG
GAGATGAAATAAATCACCAGGAATTCGTGGCTCTTGAGATGATAGAGGTGGAAACTTGA

Structures

Toxin

• AlphaFold DB: A0A0B5L026

Antitoxin

• AlphaFold DB: A0A0A6N0B1