235152

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 40586..41120 Replicon plasmid p1
Accession NZ_CP091900
Organism Enterococcus faecalis strain 101-1

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag L5I21_RS15170 Protein ID WP_002360769.1
Coordinates 40586..40861 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag L5I21_RS15175 Protein ID WP_002369771.1
Coordinates 40854..41120 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I21_RS15145 (L5I21_15145) 35783..36511 - 729 WP_002368105.1 hypothetical protein -
L5I21_RS15150 (L5I21_15150) 37161..38420 - 1260 WP_002368103.1 PcfJ domain-containing protein -
L5I21_RS15155 (L5I21_15155) 38420..39199 - 780 WP_002368102.1 Cas9 inhibitor AcrIIA9 family protein -
L5I21_RS15160 (L5I21_15160) 39430..39576 - 147 WP_002368101.1 DpnD/PcfM family protein -
L5I21_RS15165 (L5I21_15165) 39598..39783 - 186 WP_002368099.1 hypothetical protein -
L5I21_RS15170 (L5I21_15170) 40586..40861 - 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
L5I21_RS15175 (L5I21_15175) 40854..41120 - 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
L5I21_RS15180 (L5I21_15180) 41231..41815 - 585 WP_002368095.1 thermonuclease family protein -
L5I21_RS15185 (L5I21_15185) 41839..42255 - 417 WP_002368093.1 single-stranded DNA-binding protein -
L5I21_RS15190 (L5I21_15190) 42353..42847 - 495 WP_002368092.1 DnaJ domain-containing protein -
L5I21_RS15195 (L5I21_15195) 42880..43146 - 267 WP_002368091.1 hypothetical protein -
L5I21_RS15200 (L5I21_15200) 43738..44226 - 489 WP_002368088.1 hypothetical protein -
L5I21_RS15205 (L5I21_15205) 44232..45017 - 786 WP_000803546.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-III / ant(6)-Ia / erm(B) - 1..65785 65785


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-86)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T235152 WP_002360769.1 NZ_CP091900:c40861-40586 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T235152 NZ_LR134222:1819712-1819963 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9772.09 Da        Isoelectric Point: 4.4791

>AT235152 WP_002369771.1 NZ_CP091900:c41120-40854 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT235152 NZ_LR134222:1819960-1820214 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References