Detailed information of TA system TA235151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 2849..3518
• Replicon: plasmid p1
• Accession: NZ_CP091900
• Organism: Enterococcus faecalis strain 101-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: L5I21_RS14930
• Protein ID: WP_010708525.1
• Coordinates: 2849..3244 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: L5I21_RS14935
• Protein ID: WP_002331065.1
• Coordinates: 3246..3518 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I21_RS14910 (L5I21_14910)	1..1014	+	1014	WP_002387529.1	replication initiator protein A	-
L5I21_RS14915 (L5I21_14915)	1145..1813	+	669	WP_002383592.1	type II CAAX endopeptidase family protein	-
L5I21_RS14920 (L5I21_14920)	1856..2107	+	252	WP_010708530.1	hypothetical protein	-
L5I21_RS14925 (L5I21_14925)	2141..2821	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
L5I21_RS14930 (L5I21_14930)	2849..3244	-	396	WP_010708525.1	zeta toxin family protein	Toxin
L5I21_RS14935 (L5I21_14935)	3246..3518	-	273	WP_002331065.1	antitoxin	Antitoxin
L5I21_RS14940 (L5I21_14940)	3535..3744	-	210	WP_000527318.1	peptide-binding protein	-
L5I21_RS14945 (L5I21_14945)	3843..4739	-	897	WP_002334978.1	AAA family ATPase	-
L5I21_RS14950 (L5I21_14950)	5037..5609	-	573	WP_001079938.1	HTH domain-containing protein	-
L5I21_RS14955 (L5I21_14955)	5885..6679	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
L5I21_RS14960 (L5I21_14960)	6772..7314	-	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
L5I21_RS14965 (L5I21_14965)	7311..8219	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-III / ant(6)-Ia / erm(B)	-	1..65785	65785
	-	inside	IScluster/Tn	aph(3')-III / ant(6)-Ia / erm(B)	-	2141..12215	10074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T235151 WP_010708525.1 NZ_CP091900:c3244-2849 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

>T235151 NZ_LR134222:998412-998747 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT235151 WP_002331065.1 NZ_CP091900:c3518-3246 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT235151 NZ_LR134222:998164-998412 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55