Detailed information of TA system TA235118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2788998..2789569
• Replicon: chromosome
• Accession: NZ_CP091897
• Organism: Enterococcus faecalis strain 119-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5I23_RS13360
• Protein ID: WP_002354774.1
• Coordinates: 2788998..2789339 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: L5I23_RS13365
• Protein ID: WP_002354773.1
• Coordinates: 2789339..2789569 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I23_RS13355 (2785013)	2785013..2788627	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
L5I23_RS13360 (2788998)	2788998..2789339	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I23_RS13365 (2789339)	2789339..2789569	-	231	WP_002354773.1	hypothetical protein	Antitoxin
L5I23_RS13370 (2789743)	2789743..2789958	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
L5I23_RS13375 (2790097)	2790097..2791089	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
L5I23_RS13380 (2791256)	2791256..2791894	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
L5I23_RS13385 (2792579)	2792579..2794195	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T235118 WP_002354774.1 NZ_CP091897:c2789339-2788998 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T235118 NZ_LR134216:994034-994369 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT235118 WP_002354773.1 NZ_CP091897:c2789569-2789339 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT235118 NZ_LR134216:993786-994034 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812