Detailed information of TA system TA235101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2778294..2778865
• Replicon: chromosome
• Accession: NZ_CP091895
• Organism: Enterococcus faecalis strain 119-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5I25_RS13280
• Protein ID: WP_002354774.1
• Coordinates: 2778294..2778635 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: L5I25_RS13285
• Protein ID: WP_002354773.1
• Coordinates: 2778635..2778865 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I25_RS13275 (2774309)	2774309..2777923	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
L5I25_RS13280 (2778294)	2778294..2778635	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I25_RS13285 (2778635)	2778635..2778865	-	231	WP_002354773.1	hypothetical protein	Antitoxin
L5I25_RS13290 (2779039)	2779039..2779254	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
L5I25_RS13295 (2779393)	2779393..2780385	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
L5I25_RS13300 (2780552)	2780552..2781190	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
L5I25_RS13305 (2781875)	2781875..2783491	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T235101 WP_002354774.1 NZ_CP091895:c2778635-2778294 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T235101 NZ_LR134215:80176-80487 [Shimwellia blattae]
ATGCATATTATATCAAGAGCGCCGTTCGATACCGCGACCAAAATATTCCCTAATCAGGCTGCTGCACTTGATGATCTTTA
CCGGGTTCTGAAACGTGAGAATTATCGCTCACCGGATGAGATGAGAATGCAATTTCCCAGCCTGGACCGGATGAAGCTCC
GGGAAAAATGGTGGGTTATTGATGTGGGTGGTGGTCAGCTAAGGGTGATGTTCTTTGCTGATTTTGAACGGGGAAAGTTG
TTCATCAAGCATATTTCTACCCATGCTGAATACGACAAGCTGACGGACTATTACCGGAGGAACAAAGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT235101 WP_002354773.1 NZ_CP091895:c2778865-2778635 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT235101 NZ_LR134215:80484-80897 [Shimwellia blattae]
GTGAGTTACGCAGAAGCCATTAAAACTGCGCAAGCGCTGGCAACCATGTTCCCGCTGCTTGGGGGAAGCACCTCCCGTAA
AGATTATGAAGACGCTTTGCGGATGGTGGAGTATCTGGTCGAACATGAGCCGGATAGCCCGCTGATTGATATGCTGGCCG
CCAGGATAGAGAAATACGAGGATGAAGCTCCTGAATTCGCCGAGTTTAACGCGCGCATTGCCAGCGTTCCGGCGGGAGTA
TCGGTACTCAGGGTTATCATGGATCAGTATCATTTAACGCAGAGTGATTTCGAGCAGGAGATTGGTAAAAAATCCCTGGT
TAGCCGTATTTTGAGTGGTCAGCGTTCCCTGACCCTTGCGCATATCAAAGCGCTGGCCGCGCGCTTTCATATTAAGCCTG
AGCTGTTCCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812