Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ImmA-IrrE/HTH_19(antitoxin)
Location 1805396..1806089 Replicon chromosome
Accession NZ_CP091895
Organism Enterococcus faecalis strain 119-2

Toxin (Protein)


Gene name IrrE Uniprot ID S4BLV4
Locus tag L5I25_RS08860 Protein ID WP_002378467.1
Coordinates 1805745..1806089 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name ImmA Uniprot ID -
Locus tag L5I25_RS08855 Protein ID WP_002364355.1
Coordinates 1805396..1805728 (+) Length 111 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I25_RS08810 (1800868) 1800868..1801602 - 735 WP_002378463.1 ERF family protein -
L5I25_RS08815 (1801595) 1801595..1801912 - 318 WP_002357007.1 hypothetical protein -
L5I25_RS08820 (1802132) 1802132..1802686 + 555 WP_002357006.1 hypothetical protein -
L5I25_RS08825 (1803113) 1803113..1803307 - 195 WP_002378464.1 hypothetical protein -
L5I25_RS08830 (1803344) 1803344..1803553 - 210 WP_002378465.1 hypothetical protein -
L5I25_RS08835 (1803608) 1803608..1803796 + 189 WP_002357001.1 YegP family protein -
L5I25_RS08840 (1803822) 1803822..1804547 - 726 WP_002378466.1 phage regulatory protein -
L5I25_RS08845 (1804586) 1804586..1804897 - 312 WP_002381719.1 hypothetical protein -
L5I25_RS08850 (1804908) 1804908..1805084 - 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
L5I25_RS08855 (1805396) 1805396..1805728 + 333 WP_002364355.1 helix-turn-helix transcriptional regulator Antitoxin
L5I25_RS08860 (1805745) 1805745..1806089 + 345 WP_002378467.1 ImmA/IrrE family metallo-endopeptidase Toxin
L5I25_RS08865 (1806125) 1806125..1806853 + 729 WP_002378468.1 potassium channel family protein -
L5I25_RS08870 (1806953) 1806953..1808101 + 1149 WP_002378469.1 site-specific integrase -
L5I25_RS08875 (1808138) 1808138..1808572 - 435 Protein_1720 competence type IV pilus minor pilin ComGD -
L5I25_RS08880 (1808569) 1808569..1808844 - 276 WP_002356991.1 competence type IV pilus major pilin ComGC -
L5I25_RS08885 (1808844) 1808844..1809890 - 1047 WP_002369171.1 competence type IV pilus assembly protein ComGB -
L5I25_RS08890 (1809847) 1809847..1810815 - 969 WP_002364362.1 competence type IV pilus ATPase ComGA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1759669..1808545 48876


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(22-89)

Antitoxin

(5-63)


Sequences


Toxin        


Download         Length: 115 a.a.        Molecular weight: 13712.62 Da        Isoelectric Point: 5.5523

>T235096 WP_002378467.1 NZ_CP091895:1805745-1806089 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYIELYKIPSFRNKMEAEADY
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Download         Length: 345 bp

>T235096 NZ_LR134214:c2369233-2368955 [Citrobacter portucalensis]
ATGATCATGAACTTCAGGCACAAGGGATTGCGCGATTTGTTTCTTCATGGACGAACATCGGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGACTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCTGGTTATAAGCTAC
ATCCTCTGGTGGGTGAACGCGACGGCATATGGGCAATTTCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGATTACGAGGACTATCATTGA

Antitoxin


Download         Length: 111 a.a.        Molecular weight: 12691.16 Da        Isoelectric Point: 4.6307

>AT235096 WP_002364355.1 NZ_CP091895:1805396-1805728 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Download         Length: 333 bp

>AT235096 NZ_LR134214:c2368955-2368671 [Citrobacter portucalensis]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCACTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCGTCAACGGCAAGTCGACTATTAACCGGGAAAGCGGCTCTGACGCCTGAGATGGCGA
TTAAACTCTCCGTAGTGATTGGCAGTTCACCCGAAATGTGGTTAAACCTGCAAAATACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGTCTGCGTAAGCTGGCGGTACAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S4BLV4


Antitoxin

Source ID Structure

References