Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1517808..1518945 Replicon chromosome
Accession NZ_CP091895
Organism Enterococcus faecalis strain 119-2

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag L5I25_RS07325 Protein ID WP_002401483.1
Coordinates 1517808..1518671 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag L5I25_RS07330 Protein ID WP_000301765.1
Coordinates 1518673..1518945 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I25_RS07290 (1513014) 1513014..1513877 - 864 WP_002382350.1 DNA-processing protein DprA -
L5I25_RS07295 (1513938) 1513938..1514705 - 768 WP_002357481.1 ribonuclease HII -
L5I25_RS07300 (1514707) 1514707..1515558 - 852 WP_002357480.1 ribosome biogenesis GTPase YlqF -
L5I25_RS07305 (1515834) 1515834..1515950 - 117 Protein_1409 ketopantoate reductase C-terminal domain-containing protein -
L5I25_RS07310 (1516005) 1516005..1516685 - 681 WP_010710189.1 IS6-like element IS1216 family transposase -
L5I25_RS07315 (1516761) 1516761..1517030 - 270 Protein_1411 DnaJ domain-containing protein -
L5I25_RS07320 (1517050) 1517050..1517367 - 318 WP_002333463.1 hypothetical protein -
L5I25_RS07325 (1517808) 1517808..1518671 - 864 WP_002401483.1 zeta toxin family protein Toxin
L5I25_RS07330 (1518673) 1518673..1518945 - 273 WP_000301765.1 antitoxin Antitoxin
L5I25_RS07335 (1518962) 1518962..1519177 - 216 WP_002295735.1 peptide-binding protein -
L5I25_RS07340 (1519276) 1519276..1520172 - 897 WP_002387620.1 ParA family protein -
L5I25_RS07345 (1520275) 1520275..1520535 - 261 Protein_1417 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
L5I25_RS07350 (1520705) 1520705..1521442 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
L5I25_RS07355 (1521567) 1521567..1521650 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
L5I25_RS07360 (1521699) 1521699..1521938 - 240 WP_000635249.1 peptide-binding protein -
L5I25_RS07365 (1522058) 1522058..1523890 - 1833 WP_233050403.1 type IA DNA topoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) - 1516005..1525299 9294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T235095 WP_002401483.1 NZ_CP091895:c1518671-1517808 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T235095 NZ_LR134214:1951740-1951843 [Citrobacter portucalensis]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT235095 WP_000301765.1 NZ_CP091895:c1518945-1518673 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT235095 NZ_LR134214:c1951848-1951705 [Citrobacter portucalensis]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References