Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 146255..146826 Replicon plasmid p1
Accession NZ_CP091893
Organism Enterococcus faecalis strain 133-1

Toxin (Protein)


Gene name mazF Uniprot ID S4H3R9
Locus tag L5I22_RS15045 Protein ID WP_010784114.1
Coordinates 146485..146826 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag L5I22_RS15040 Protein ID WP_002362431.1
Coordinates 146255..146485 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I22_RS15000 (L5I22_15000) 141522..141944 + 423 WP_010815591.1 CsbD family protein -
L5I22_RS15005 (L5I22_15005) 142108..142674 + 567 WP_002360874.1 alkaline shock response membrane anchor protein AmaP -
L5I22_RS15010 (L5I22_15010) 142691..142888 + 198 WP_002360877.1 DUF2273 domain-containing protein -
L5I22_RS15015 (L5I22_15015) 142915..143451 + 537 WP_002360878.1 Asp23/Gls24 family envelope stress response protein -
L5I22_RS15020 (L5I22_15020) 143580..144638 - 1059 WP_002417138.1 hypothetical protein -
L5I22_RS15025 (L5I22_15025) 144663..144905 - 243 WP_002360880.1 GlsB/YeaQ/YmgE family stress response membrane protein -
L5I22_RS15030 (L5I22_15030) 145110..145352 + 243 WP_002360881.1 hypothetical protein -
L5I22_RS15035 (L5I22_15035) 145438..145644 + 207 Protein_173 transposase -
L5I22_RS15040 (L5I22_15040) 146255..146485 + 231 WP_002362431.1 hypothetical protein Antitoxin
L5I22_RS15045 (L5I22_15045) 146485..146826 + 342 WP_010784114.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5I22_RS15050 (L5I22_15050) 146938..147876 + 939 WP_002362433.1 hypothetical protein -
L5I22_RS15055 (L5I22_15055) 148141..148743 + 603 WP_002362434.1 Fic family protein -
L5I22_RS15060 (L5I22_15060) 148882..150005 + 1124 WP_154805971.1 IS3 family transposase -
L5I22_RS15065 (L5I22_15065) 150143..151426 + 1284 WP_002405123.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia prgB/asc10 1..172742 172742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13270.47 Da        Isoelectric Point: 7.9750

>T235088 WP_010784114.1 NZ_CP091893:146485-146826 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T235088 NZ_LR134213:1929520-1929622 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT235088 WP_002362431.1 NZ_CP091893:146255-146485 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT235088 NZ_LR134213:c1929629-1929484 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S4H3R9

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References