Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | mazEF/PRK09907(toxin) |
Location | 146255..146826 | Replicon | plasmid p1 |
Accession | NZ_CP091893 | ||
Organism | Enterococcus faecalis strain 133-1 |
Toxin (Protein)
Gene name | mazF | Uniprot ID | S4H3R9 |
Locus tag | L5I22_RS15045 | Protein ID | WP_010784114.1 |
Coordinates | 146485..146826 (+) | Length | 114 a.a. |
Antitoxin (Protein)
Gene name | mazE | Uniprot ID | R3KHK9 |
Locus tag | L5I22_RS15040 | Protein ID | WP_002362431.1 |
Coordinates | 146255..146485 (+) | Length | 77 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
L5I22_RS15000 (L5I22_15000) | 141522..141944 | + | 423 | WP_010815591.1 | CsbD family protein | - |
L5I22_RS15005 (L5I22_15005) | 142108..142674 | + | 567 | WP_002360874.1 | alkaline shock response membrane anchor protein AmaP | - |
L5I22_RS15010 (L5I22_15010) | 142691..142888 | + | 198 | WP_002360877.1 | DUF2273 domain-containing protein | - |
L5I22_RS15015 (L5I22_15015) | 142915..143451 | + | 537 | WP_002360878.1 | Asp23/Gls24 family envelope stress response protein | - |
L5I22_RS15020 (L5I22_15020) | 143580..144638 | - | 1059 | WP_002417138.1 | hypothetical protein | - |
L5I22_RS15025 (L5I22_15025) | 144663..144905 | - | 243 | WP_002360880.1 | GlsB/YeaQ/YmgE family stress response membrane protein | - |
L5I22_RS15030 (L5I22_15030) | 145110..145352 | + | 243 | WP_002360881.1 | hypothetical protein | - |
L5I22_RS15035 (L5I22_15035) | 145438..145644 | + | 207 | Protein_173 | transposase | - |
L5I22_RS15040 (L5I22_15040) | 146255..146485 | + | 231 | WP_002362431.1 | hypothetical protein | Antitoxin |
L5I22_RS15045 (L5I22_15045) | 146485..146826 | + | 342 | WP_010784114.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
L5I22_RS15050 (L5I22_15050) | 146938..147876 | + | 939 | WP_002362433.1 | hypothetical protein | - |
L5I22_RS15055 (L5I22_15055) | 148141..148743 | + | 603 | WP_002362434.1 | Fic family protein | - |
L5I22_RS15060 (L5I22_15060) | 148882..150005 | + | 1124 | WP_154805971.1 | IS3 family transposase | - |
L5I22_RS15065 (L5I22_15065) | 150143..151426 | + | 1284 | WP_002405123.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | erm(B) / aph(3')-III / ant(6)-Ia | prgB/asc10 | 1..172742 | 172742 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 13270.47 Da Isoelectric Point: 7.9750
>T235088 WP_010784114.1 NZ_CP091893:146485-146826 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF
Download Length: 342 bp
>T235088 NZ_LR134213:1929520-1929622 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 77 a.a. Molecular weight: 8510.42 Da Isoelectric Point: 4.3855
>AT235088 WP_002362431.1 NZ_CP091893:146255-146485 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF
Download Length: 231 bp
>AT235088 NZ_LR134213:c1929629-1929484 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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