Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 69182..69878 Replicon plasmid p1
Accession NZ_CP091893
Organism Enterococcus faecalis strain 133-1

Toxin (Protein)


Gene name zeta Uniprot ID A0A828ZQ58
Locus tag L5I22_RS14605 Protein ID WP_002334977.1
Coordinates 69182..69604 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL01
Locus tag L5I22_RS14610 Protein ID WP_002331065.1
Coordinates 69606..69878 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I22_RS14560 (L5I22_14560) 65031..65645 - 615 WP_000395511.1 bacitracin resistance transcriptional activator BcrR -
L5I22_RS14565 (L5I22_14565) 65635..65961 - 327 WP_002387760.1 DNA-binding protein -
L5I22_RS14570 (L5I22_14570) 65998..66162 + 165 WP_001808810.1 helix-turn-helix transcriptional regulator -
L5I22_RS14575 (L5I22_14575) 66201..66881 + 681 WP_000191454.1 IS6-like element ISEnfa1 family transposase -
L5I22_RS14580 (L5I22_14580) 66935..66997 - 63 Protein_82 rRNA adenine methyltransferase -
L5I22_RS14585 (L5I22_14585) 67002..67739 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
L5I22_RS14590 (L5I22_14590) 67864..67947 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
L5I22_RS14595 (L5I22_14595) 68070..68237 - 168 Protein_85 peptide-binding protein -
L5I22_RS14600 (L5I22_14600) 68448..69128 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
L5I22_RS14605 (L5I22_14605) 69182..69604 - 423 WP_002334977.1 zeta toxin family protein Toxin
L5I22_RS14610 (L5I22_14610) 69606..69878 - 273 WP_002331065.1 antitoxin Antitoxin
L5I22_RS14615 (L5I22_14615) 69895..70104 - 210 WP_000527318.1 peptide-binding protein -
L5I22_RS14620 (L5I22_14620) 70203..71099 - 897 WP_002334978.1 AAA family ATPase -
L5I22_RS14625 (L5I22_14625) 71397..71969 - 573 WP_001079938.1 HTH domain-containing protein -
L5I22_RS14630 (L5I22_14630) 72245..73039 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
L5I22_RS14635 (L5I22_14635) 73132..73674 - 543 WP_000627290.1 streptothricin N-acetyltransferase Sat4 -
L5I22_RS14640 (L5I22_14640) 73671..74579 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia prgB/asc10 1..172742 172742
- inside IScluster/Tn erm(B) / aph(3')-III / ant(6)-Ia - 61482..74579 13097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-134)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15667.64 Da        Isoelectric Point: 5.2420

>T235087 WP_002334977.1 NZ_CP091893:c69604-69182 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSGSVAKF

Download         Length: 423 bp

>T235087 NZ_LR134213:798388-798798 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10733.17 Da        Isoelectric Point: 4.4865

>AT235087 WP_002331065.1 NZ_CP091893:c69878-69606 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Download         Length: 273 bp

>AT235087 NZ_LR134213:798141-798407 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZQ58

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZL55

References