Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 40521..41055 Replicon plasmid p1
Accession NZ_CP091893
Organism Enterococcus faecalis strain 133-1

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag L5I22_RS14420 Protein ID WP_002360769.1
Coordinates 40521..40796 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag L5I22_RS14425 Protein ID WP_002369771.1
Coordinates 40789..41055 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I22_RS14395 (L5I22_14395) 35711..36439 - 729 WP_002383622.1 hypothetical protein -
L5I22_RS14400 (L5I22_14400) 37088..38347 - 1260 WP_024041582.1 PcfJ domain-containing protein -
L5I22_RS14405 (L5I22_14405) 38347..39126 - 780 WP_142958213.1 Cas9 inhibitor AcrIIA9 family protein -
L5I22_RS14410 (L5I22_14410) 39357..39548 - 192 WP_025186526.1 DpnD/PcfM family protein -
L5I22_RS14415 (L5I22_14415) 39533..39718 - 186 WP_002365983.1 hypothetical protein -
L5I22_RS14420 (L5I22_14420) 40521..40796 - 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
L5I22_RS14425 (L5I22_14425) 40789..41055 - 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
L5I22_RS14430 (L5I22_14430) 41158..41406 - 249 WP_002369773.1 hypothetical protein -
L5I22_RS14435 (L5I22_14435) 41403..41987 - 585 WP_024041579.1 thermonuclease family protein -
L5I22_RS14440 (L5I22_14440) 42011..42427 - 417 WP_002368093.1 single-stranded DNA-binding protein -
L5I22_RS14445 (L5I22_14445) 42524..43018 - 495 WP_010822509.1 DnaJ domain-containing protein -
L5I22_RS14450 (L5I22_14450) 43051..43317 - 267 WP_002365974.1 hypothetical protein -
L5I22_RS14455 (L5I22_14455) 43909..44397 - 489 WP_024041577.1 hypothetical protein -
L5I22_RS14460 (L5I22_14460) 44403..45188 - 786 WP_000803546.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia prgB/asc10 1..172742 172742


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-87)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T235086 WP_002360769.1 NZ_CP091893:c40796-40521 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T235086 NZ_LR134213:201457-201942 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9804.15 Da        Isoelectric Point: 4.4791

>AT235086 WP_002369771.1 NZ_CP091893:c41055-40789 [Enterococcus faecalis]
MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT235086 NZ_LR134213:201203-201469 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References