Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2832313..2832884 Replicon chromosome
Accession NZ_CP091892
Organism Enterococcus faecalis strain 133-1

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag L5I22_RS13610 Protein ID WP_002360937.1
Coordinates 2832313..2832654 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag L5I22_RS13615 Protein ID WP_002354773.1
Coordinates 2832654..2832884 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I22_RS13605 (2828328) 2828328..2831942 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
L5I22_RS13610 (2832313) 2832313..2832654 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5I22_RS13615 (2832654) 2832654..2832884 - 231 WP_002354773.1 hypothetical protein Antitoxin
L5I22_RS13620 (2833291) 2833291..2833506 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
L5I22_RS13625 (2833645) 2833645..2834637 + 993 WP_023894581.1 biotin--[acetyl-CoA-carboxylase] ligase -
L5I22_RS13630 (2834901) 2834901..2835539 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
L5I22_RS13635 (2836225) 2836225..2837841 + 1617 WP_047648929.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T235081 WP_002360937.1 NZ_CP091892:c2832654-2832313 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T235081 NZ_LR134209:c4612216-4611905 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT235081 WP_002354773.1 NZ_CP091892:c2832884-2832654 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT235081 NZ_LR134209:c4611904-4611614 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References