Detailed information of TA system TA235056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2415705..2416400
• Replicon: chromosome
• Accession: NZ_CP091892
• Organism: Enterococcus faecalis strain 133-1

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: L5I22_RS11700
• Protein ID: WP_002388462.1
• Coordinates: 2416056..2416400 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: L5I22_RS11695
• Protein ID: WP_002358111.1
• Coordinates: 2415705..2416037 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I22_RS11645 (2411177)	2411177..2411992	-	816	WP_002417285.1	PD-(D/E)XK nuclease-like domain-containing protein	-
L5I22_RS11650 (2411955)	2411955..2412920	-	966	WP_002415270.1	RecT family recombinase	-
L5I22_RS11655 (2413021)	2413021..2413245	-	225	WP_137193403.1	hypothetical protein	-
L5I22_RS11660 (2413242)	2413242..2413541	-	300	WP_137278689.1	hypothetical protein	-
L5I22_RS11665 (2413608)	2413608..2413787	-	180	WP_002367283.1	hypothetical protein	-
L5I22_RS11670 (2413822)	2413822..2414091	-	270	WP_002365131.1	hypothetical protein	-
L5I22_RS11675 (2414167)	2414167..2414481	+	315	WP_010776102.1	hypothetical protein	-
L5I22_RS15435 (2414462)	2414462..2414590	-	129	WP_010776103.1	hypothetical protein	-
L5I22_RS11680 (2414738)	2414738..2414920	+	183	WP_122634212.1	hypothetical protein	-
L5I22_RS11685 (2414912)	2414912..2415214	-	303	WP_104802090.1	hypothetical protein	-
L5I22_RS11690 (2415227)	2415227..2415409	-	183	WP_104802091.1	hypothetical protein	-
L5I22_RS11695 (2415705)	2415705..2416037	+	333	WP_002358111.1	helix-turn-helix transcriptional regulator	Antitoxin
L5I22_RS11700 (2416056)	2416056..2416400	+	345	WP_002388462.1	ImmA/IrrE family metallo-endopeptidase	Toxin
L5I22_RS11705 (2416459)	2416459..2417187	+	729	WP_002373912.1	potassium channel family protein	-
L5I22_RS11710 (2417341)	2417341..2418522	+	1182	WP_174131425.1	site-specific integrase	-
L5I22_RS11715 (2418625)	2418625..2418774	-	150	WP_002356321.1	50S ribosomal protein L33	-
L5I22_RS11720 (2418900)	2418900..2421038	-	2139	WP_024041710.1	penicillin-binding transpeptidase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2383865..2418522	34657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13654.62 Da
• Isoelectric Point: 5.2241

>T235056 WP_002388462.1 NZ_CP091892:2416056-2416400 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMELYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEVYLK

Nucleotide

• Length: 345 bp

>T235056 NZ_LR134201:1095064-1095231 [Cedecea lapagei]
ATGCAACTGGCAGGGTTGTATGGCCTGAAAGTTCCAGGATGCGAAATCATCACAACCCGTAATATTAAGGCTCTGGCCGT
GGAGCGTTTCGATCGCCGTTTTGTGGATAATAATCGCTGGATTGCCCGTCTTCCGCAGGAGGATATTTGCCAGAAAATGT
GGTTTTAG

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12720.11 Da
• Isoelectric Point: 4.5116

>AT235056 WP_002358111.1 NZ_CP091892:2415705-2416037 [Enterococcus faecalis]
MNTYEIIKDLAKEKGISIRQLEINFGYSNGYIGSWKRQTPNSNELSRLADYFDVSVDYLLGRTDNTKATDEKKFDDLDDV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT235056 NZ_LR134201:1094801-1095049 [Cedecea lapagei]
ATGAAAATTACTTCCCCGGAACAATTGGCTATTTTCCTGAAGGATGCTCGCAAACAGGGTAAACAAACACAGGGGGCAGT
GGCAGAGAAAGTCGGCTTACGCCAGGATACCGTCTCAAAATTTGAGAATAACCCAGCCAAAACTCAGATTGATACATTAT
TTAAAATACTGTCGTCTATGAATATGGAGCTTCATATTGAGACGAAATCCAGCCAGGGGAGCTCTGAAGTCTGGAAAGAG
GAGTGGTAA