Detailed information of TA system TA023505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2210280..2210863
• Replicon: chromosome
• Accession: NC_011901
• Organism: Thioalkalivibrio sulfidophilus HL-EbGr7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TGR7_RS10435
• Protein ID: WP_041441613.1
• Coordinates: 2210489..2210863 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B8GTR7
• Locus tag: TGR7_RS10430
• Protein ID: WP_012638640.1
• Coordinates: 2210280..2210501 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TGR7_RS10400	2205322..2205750	+	429	WP_012638635.1	type II toxin-antitoxin system HicB family antitoxin	-
TGR7_RS10410	2206366..2206644	+	279	WP_012638636.1	DUF1778 domain-containing protein	-
TGR7_RS10415	2206656..2207159	+	504	WP_012638637.1	GNAT family N-acetyltransferase	-
TGR7_RS10420	2207315..2208787	-	1473	WP_148211493.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
TGR7_RS10425	2208891..2210126	+	1236	WP_012638639.1	O-antigen ligase family protein	-
TGR7_RS10430	2210280..2210501	+	222	WP_012638640.1	ribbon-helix-helix domain-containing protein	Antitoxin
TGR7_RS10435	2210489..2210863	+	375	WP_041441613.1	type II toxin-antitoxin system VapC family toxin	Toxin
TGR7_RS10440	2210958..2212376	-	1419	WP_012638642.1	undecaprenyl-phosphate glucose phosphotransferase	-
TGR7_RS10445	2212485..2212892	-	408	WP_012638643.1	type II toxin-antitoxin system VapC family toxin	-
TGR7_RS10450	2212892..2213152	-	261	WP_012638644.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TGR7_RS10455	2213221..2214186	-	966	WP_012638645.1	transposase	-
TGR7_RS10460	2214245..2214619	-	375	WP_012638646.1	DNA-binding protein	-
TGR7_RS10465	2214637..2214930	-	294	WP_012638647.1	type II toxin-antitoxin system HigB family toxin	-
TGR7_RS10470	2215266..2215862	+	597	WP_012638648.1	Uma2 family endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fcl / gmd	2205132..2237146	32014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.03 Da
• Isoelectric Point: 4.5407

>T23505 WP_041441613.1 NC_011901:2210489-2210863 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
VDSMKAVFDTNILVDYLNGLPQAAEELNRYNQRLISQITWMEVMAGCRDDQEEQLVRGFLATFDVVPIDAQVAETAVILR
RTNRLRLPDAIILATAECSHALLVTRDTKDFPSDTPGIRVPYRL

Nucleotide

• Length: 375 bp

>T23505 NC_011901:2210489-2210863 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
GTGGACTCGATGAAGGCGGTCTTCGACACCAACATACTGGTGGACTACCTCAATGGACTTCCCCAGGCTGCCGAGGAATT
GAATCGTTACAACCAACGGTTGATCAGCCAGATCACCTGGATGGAAGTCATGGCAGGCTGCCGGGACGACCAGGAAGAGC
AATTGGTACGCGGCTTCCTTGCCACTTTCGATGTGGTACCGATCGACGCACAGGTTGCCGAAACAGCGGTCATACTCCGA
CGCACCAACCGCCTCAGACTCCCGGACGCCATCATCCTCGCCACAGCCGAATGCAGTCACGCGCTCCTGGTGACCCGCGA
CACGAAGGATTTTCCTTCCGACACCCCGGGGATACGGGTGCCGTATAGGTTGTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8477.57 Da
• Isoelectric Point: 5.8650

>AT23505 WP_012638640.1 NC_011901:2210280-2210501 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MRTVVDLPDEELQAIKALAKREKISQAEAIRRAVRAYLHARPAPDKESPAFGLWEGREEGIQYQDTLRDEWTR

Nucleotide

• Length: 222 bp

>AT23505 NC_011901:2210280-2210501 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGCGCACTGTAGTCGATCTTCCCGACGAAGAACTCCAGGCCATCAAGGCTCTGGCCAAGCGGGAGAAAATCTCCCAGGC
TGAGGCCATCCGGCGCGCCGTCCGGGCATACCTCCACGCCCGCCCGGCACCCGATAAAGAATCACCCGCATTCGGCCTAT
GGGAGGGGCGGGAGGAAGGAATTCAGTATCAGGACACACTCAGGGATGAGTGGACTCGATGA

Structures

Antitoxin

• AlphaFold DB: B8GTR7