Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | omega-epsilon-zeta/zeta-epsilon |
| Location | 49815..50952 | Replicon | plasmid p2 |
| Accession | NZ_CP091891 | ||
| Organism | Enterococcus faecalis strain 142-1 | ||
Toxin (Protein)
| Gene name | zeta | Uniprot ID | Q54944 |
| Locus tag | L5I18_RS16615 | Protein ID | WP_001284311.1 |
| Coordinates | 50089..50952 (+) | Length | 288 a.a. |
Antitoxin (Protein)
| Gene name | epsilon | Uniprot ID | R2XCR7 |
| Locus tag | L5I18_RS16610 | Protein ID | WP_000301765.1 |
| Coordinates | 49815..50087 (+) | Length | 91 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L5I18_RS16585 (L5I18_16585) | 45547..45717 | + | 171 | WP_000713594.1 | hypothetical protein | - |
| L5I18_RS16590 (L5I18_16590) | 45731..46348 | + | 618 | WP_001062589.1 | recombinase family protein | - |
| L5I18_RS16595 (L5I18_16595) | 46348..48492 | + | 2145 | WP_000108741.1 | type IA DNA topoisomerase | - |
| L5I18_RS16600 (L5I18_16600) | 48595..49491 | + | 897 | WP_001835294.1 | ParA family protein | - |
| L5I18_RS16605 (L5I18_16605) | 49583..49798 | + | 216 | WP_001835296.1 | peptide-binding protein | - |
| L5I18_RS16610 (L5I18_16610) | 49815..50087 | + | 273 | WP_000301765.1 | antitoxin | Antitoxin |
| L5I18_RS16615 (L5I18_16615) | 50089..50952 | + | 864 | WP_001284311.1 | zeta toxin family protein | Toxin |
| L5I18_RS16620 (L5I18_16620) | 51141..51719 | + | 579 | WP_238464146.1 | TetR/AcrR family transcriptional regulator | - |
| L5I18_RS16625 (L5I18_16625) | 51751..52194 | + | 444 | WP_032490933.1 | small multi-drug export protein | - |
| L5I18_RS16630 (L5I18_16630) | 52347..52718 | + | 372 | WP_238464148.1 | transposase | - |
| L5I18_RS16635 (L5I18_16635) | 52746..53087 | - | 342 | WP_238464150.1 | hypothetical protein | - |
| L5I18_RS16640 (L5I18_16640) | 53105..53305 | - | 201 | WP_238464151.1 | hypothetical protein | - |
| L5I18_RS16645 (L5I18_16645) | 53832..54569 | - | 738 | WP_128888465.1 | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) | - |
| L5I18_RS16650 (L5I18_16650) | 54694..54777 | - | 84 | WP_001814874.1 | 23S rRNA methyltransferase attenuation leader peptide | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') / erm(B) | - | 1..59470 | 59470 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 288 a.a. Molecular weight: 32404.02 Da Isoelectric Point: 6.9964
>T235049 WP_001284311.1 NZ_CP091891:50089-50952 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI
Download Length: 864 bp
>T235049 NZ_LR134196:3880415-3880633 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA
Antitoxin
Download Length: 91 a.a. Molecular weight: 10721.25 Da Isoelectric Point: 6.4676
>AT235049 WP_000301765.1 NZ_CP091891:49815-50087 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA
Download Length: 273 bp
>AT235049 NZ_LR134196:3880014-3880388 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| PDB | 1GVN | |
| PDB | 3Q8X |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829F0A3 |