Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 3059019..3059590 Replicon chromosome
Accession NZ_CP091889
Organism Enterococcus faecalis strain 142-1

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag L5I18_RS15360 Protein ID WP_002360937.1
Coordinates 3059019..3059360 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3JGB1
Locus tag L5I18_RS15365 Protein ID WP_002354773.1
Coordinates 3059360..3059590 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5I18_RS15355 (3055034) 3055034..3058648 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
L5I18_RS15360 (3059019) 3059019..3059360 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5I18_RS15365 (3059360) 3059360..3059590 - 231 WP_002354773.1 hypothetical protein Antitoxin
L5I18_RS15370 (3059997) 3059997..3060212 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
L5I18_RS15375 (3060351) 3060351..3061343 + 993 WP_023894581.1 biotin--[acetyl-CoA-carboxylase] ligase -
L5I18_RS15380 (3061607) 3061607..3062245 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
L5I18_RS15385 (3062931) 3062931..3064547 + 1617 WP_047648929.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T235040 WP_002360937.1 NZ_CP091889:c3059360-3059019 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T235040 NZ_LR134195:c2395813-2395340 [Raoultella ornithinolytica]
ATGATTTTCTATCGTCTGGTTACCGGCCGTTACGCCAGCGAAGCCTGGACGGGAAGCGGGGCCAACCAGTACGGCGGGCG
CTGGAATCACCAGGGACACCCGGCGGTCTATGTGTCCACTTCCATTGCCCTGGCGTCACTGGAGATCCTGGTTCACGTCA
AAAATGAGGCAATCCTCAACCAGTATCAGCTGTATAGTATCGAGATACCGGACAGTGAAGTGGAATCCCTCGATAAACAG
TGGTTACCGGATAACTGGCAAGAAAATCCGATGCCCGTTTCCACAATGGATCTGGGCACCGGCTGGCTACAGTCGGGCAG
CGGTCTGGCGCTGATGCTTCCCTCCTGCATTATTCCCTATGAAAACAACGCCATTTTAAACCCGCTGCATCCCGCGTTCA
GCGAGGCCCTGGCCTCCGTGCGTCAGTATCCCTTTACCTTTGATCCCCGCCTCGCACGCTCCACGCTCCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.67 Da        Isoelectric Point: 4.3313

>AT235040 WP_002354773.1 NZ_CP091889:c3059590-3059360 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT235040 NZ_LR134195:c2396253-2395810 [Raoultella ornithinolytica]
ATGAAAACCTGGGTTCCTGCAGAGCAACCTGCCGATAATGCGCTGTGGCGCTATGCCGGGCTACCGGCGAATCGGGGTAT
GCAGTTGATTGAGCTGCTGAGTATGGGATTACCGGTCAGCATTCTGGATAATATTCATGAGTGGACGGAAATGTCGAAAA
CCGACATTCTGCGCATCACCGGGATTAATGAGCGCAACGTTGCCCGGCGCAAGAGCGCGGGCGGCACGTTGACGCCAGGC
GAAAGCGAACGTGTGGCGCGTTTTGTCAGGGTCCTGGATACCGCCGTCGACTATTTTGGCAGCAAGCAGGATGCCTACGA
CTGGCTGCAGTCGCCGGTCAGAGGACTGGGCAATGTCGCCCCCATCGACCTGATTGCTACCGAGAGCGGCGCGCTGGAAG
TAACAGACCTGATTGGTCGCCTGGAACACGGCGTCTTTGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2A812

References