Detailed information of TA system TA023504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 2206366..2207159
• Replicon: chromosome
• Accession: NC_011901
• Organism: Thioalkalivibrio sulfidophilus HL-EbGr7

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B8GTR4
• Locus tag: TGR7_RS10415
• Protein ID: WP_012638637.1
• Coordinates: 2206656..2207159 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: B8GTR3
• Locus tag: TGR7_RS10410
• Protein ID: WP_012638636.1
• Coordinates: 2206366..2206644 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TGR7_RS10390	2202690..2204867	+	2178	WP_012638633.1	1,4-alpha-glucan branching protein GlgB	-
TGR7_RS10395	2205132..2205317	+	186	WP_012638634.1	type II toxin-antitoxin system HicA family toxin	-
TGR7_RS10400	2205322..2205750	+	429	WP_012638635.1	type II toxin-antitoxin system HicB family antitoxin	-
TGR7_RS10410	2206366..2206644	+	279	WP_012638636.1	DUF1778 domain-containing protein	Antitoxin
TGR7_RS10415	2206656..2207159	+	504	WP_012638637.1	GNAT family N-acetyltransferase	Toxin
TGR7_RS10420	2207315..2208787	-	1473	WP_148211493.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
TGR7_RS10425	2208891..2210126	+	1236	WP_012638639.1	O-antigen ligase family protein	-
TGR7_RS10430	2210280..2210501	+	222	WP_012638640.1	ribbon-helix-helix domain-containing protein	-
TGR7_RS10435	2210489..2210863	+	375	WP_041441613.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fcl / gmd	2205132..2237146	32014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18326.16 Da
• Isoelectric Point: 7.2632

>T23504 WP_012638637.1 NC_011901:2206656-2207159 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MASPYAPVRKLAATDQVDAFDCDQAALNQFLQRYALVNQKANSAQTYVCCQGDVVVGLYSLAVGSVDPEAAPSRVMKGLA
RHPVPVMILARLAVDKDHQRKGLGQALLKDALLRTAQAADIAGIRCLLVHAKDDAARQWYESWEFEPSPTDPYHLFLMLK
DLKGMLS

Nucleotide

• Length: 504 bp

>T23504 NC_011901:2206656-2207159 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGGCGTCGCCCTATGCACCCGTTCGCAAGCTTGCAGCAACGGATCAAGTCGATGCGTTCGACTGCGACCAAGCCGCGCT
GAACCAGTTCCTGCAGCGCTACGCGCTCGTCAACCAGAAGGCCAACAGCGCGCAGACCTACGTCTGCTGCCAAGGTGACG
TGGTGGTCGGCCTCTACAGCCTCGCCGTCGGCAGCGTCGATCCGGAAGCCGCTCCGTCGAGGGTGATGAAGGGGCTAGCG
CGTCACCCAGTACCGGTCATGATCCTGGCCCGGCTCGCGGTGGACAAGGACCATCAACGCAAAGGCCTGGGCCAGGCATT
GCTCAAGGATGCGCTGCTGCGCACGGCGCAGGCCGCCGACATCGCCGGTATCCGCTGCCTGCTGGTACATGCCAAGGACG
ACGCAGCGCGGCAGTGGTACGAGTCCTGGGAATTCGAGCCCAGTCCGACCGATCCTTATCACCTCTTCCTGATGCTCAAA
GATCTCAAGGGCATGTTGAGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.85 Da
• Isoelectric Point: 10.0988

>AT23504 WP_012638636.1 NC_011901:2206366-2206644 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MSTLNLPKTERIDVRASTPVKQLLQEAARACHKNVSEFLLDAGVTAAAQTLADRRQFVLDEAQWQAFQEALDRPVQSKPR
LKKLLREPGVLG

Nucleotide

• Length: 279 bp

>AT23504 NC_011901:2206366-2206644 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGAGCACGCTGAATCTTCCCAAGACCGAGCGCATTGACGTCCGTGCAAGCACGCCGGTCAAGCAACTGCTGCAGGAAGC
CGCGCGCGCCTGCCACAAGAACGTCAGCGAGTTCCTGCTCGACGCGGGCGTGACGGCTGCCGCGCAGACGCTGGCGGACC
GTCGCCAGTTCGTACTGGACGAGGCGCAGTGGCAGGCGTTCCAGGAAGCGCTGGATCGTCCGGTGCAAAGCAAGCCGCGC
CTGAAGAAGCTGCTGCGTGAGCCCGGGGTGCTGGGTTGA

Structures

Toxin

• AlphaFold DB: B8GTR4

Antitoxin

• AlphaFold DB: B8GTR3