Detailed information of TA system TA023503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2205132..2205750
• Replicon: chromosome
• Accession: NC_011901
• Organism: Thioalkalivibrio sulfidophilus HL-EbGr7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B8GTR1
• Locus tag: TGR7_RS10395
• Protein ID: WP_012638634.1
• Coordinates: 2205132..2205317 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B8GTR2
• Locus tag: TGR7_RS10400
• Protein ID: WP_012638635.1
• Coordinates: 2205322..2205750 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TGR7_RS10385	2201257..2202522	-	1266	WP_012638632.1	glucose-1-phosphate adenylyltransferase	-
TGR7_RS10390	2202690..2204867	+	2178	WP_012638633.1	1,4-alpha-glucan branching protein GlgB	-
TGR7_RS10395	2205132..2205317	+	186	WP_012638634.1	type II toxin-antitoxin system HicA family toxin	Toxin
TGR7_RS10400	2205322..2205750	+	429	WP_012638635.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
TGR7_RS10410	2206366..2206644	+	279	WP_012638636.1	DUF1778 domain-containing protein	-
TGR7_RS10415	2206656..2207159	+	504	WP_012638637.1	GNAT family N-acetyltransferase	-
TGR7_RS10420	2207315..2208787	-	1473	WP_148211493.1	mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase	-
TGR7_RS10425	2208891..2210126	+	1236	WP_012638639.1	O-antigen ligase family protein	-
TGR7_RS10430	2210280..2210501	+	222	WP_012638640.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fcl / gmd	2205132..2237146	32014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6834.03 Da
• Isoelectric Point: 11.3261

>T23503 WP_012638634.1 NC_011901:2205132-2205317 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MDSRTLIKELKEAGWVEVRVRGSHHQFAHPDRPGAVLTIPHPKKDLGKGLVMAIRKQAGLR

Nucleotide

• Length: 186 bp

>T23503 NC_011901:2205132-2205317 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGGACAGCAGGACGCTGATCAAGGAGCTCAAGGAAGCGGGCTGGGTTGAGGTACGCGTACGCGGCAGTCATCACCAGTT
TGCCCACCCCGATCGCCCCGGCGCCGTACTGACCATACCCCACCCCAAGAAAGATCTTGGCAAGGGTCTTGTCATGGCCA
TCCGCAAACAGGCAGGTCTGAGATAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15451.55 Da
• Isoelectric Point: 4.4377

>AT23503 WP_012638635.1 NC_011901:2205322-2205750 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MRYPILIEPGSNSTAYSVVVPDLPGCYSAGDTLDEAIENAEEAILMHLEALIDAGESLPEPSSIEMQLNNPAYKDWIRAM
VDIDMSRIQGPAQRINITIPKRVLRAIDAAAARAHETRSGFLARAGLKAAQLQEMEKTTGSE

Nucleotide

• Length: 429 bp

>AT23503 NC_011901:2205322-2205750 [Thioalkalivibrio sulfidiphilus HL-EbGr7]
ATGCGATACCCCATCCTGATCGAACCCGGATCAAATTCCACAGCCTACAGCGTGGTAGTCCCAGATCTTCCTGGGTGCTA
TTCCGCAGGCGATACGCTAGACGAAGCGATCGAGAATGCCGAGGAAGCCATCCTCATGCATCTGGAAGCCCTGATCGATG
CCGGCGAGTCCCTGCCCGAACCCAGCAGCATTGAGATGCAGCTCAACAACCCCGCCTACAAGGACTGGATCAGGGCCATG
GTGGACATCGACATGAGCCGCATTCAGGGCCCTGCCCAGCGCATCAATATCACTATCCCCAAAAGGGTCCTGCGCGCCAT
CGACGCAGCTGCCGCCCGCGCCCACGAGACCCGCTCCGGCTTTCTTGCCAGGGCCGGGCTCAAGGCGGCACAGTTGCAAG
AAATGGAGAAAACGACCGGGAGTGAGTAG

Structures

Toxin

• AlphaFold DB: B8GTR1

Antitoxin

• AlphaFold DB: B8GTR2