Detailed information of TA system TA235026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 2007455..2008148
• Replicon: chromosome
• Accession: NZ_CP091889
• Organism: Enterococcus faecalis strain 142-1

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: S4BLV4
• Locus tag: L5I18_RS10295
• Protein ID: WP_002378467.1
• Coordinates: 2007804..2008148 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: L5I18_RS10290
• Protein ID: WP_002364355.1
• Coordinates: 2007455..2007787 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I18_RS10245 (2002927)	2002927..2003661	-	735	WP_002381724.1	ERF family protein	-
L5I18_RS10250 (2003654)	2003654..2003971	-	318	WP_002401330.1	hypothetical protein	-
L5I18_RS10255 (2004191)	2004191..2004745	+	555	WP_002357006.1	hypothetical protein	-
L5I18_RS10260 (2005172)	2005172..2005366	-	195	WP_002381722.1	hypothetical protein	-
L5I18_RS10265 (2005403)	2005403..2005612	-	210	WP_002381721.1	hypothetical protein	-
L5I18_RS10270 (2005667)	2005667..2005855	+	189	WP_002357001.1	YegP family protein	-
L5I18_RS10275 (2005881)	2005881..2006606	-	726	WP_002381720.1	phage regulatory protein	-
L5I18_RS10280 (2006645)	2006645..2006956	-	312	WP_002381719.1	hypothetical protein	-
L5I18_RS10285 (2006967)	2006967..2007143	-	177	WP_002364354.1	helix-turn-helix transcriptional regulator	-
L5I18_RS10290 (2007455)	2007455..2007787	+	333	WP_002364355.1	helix-turn-helix transcriptional regulator	Antitoxin
L5I18_RS10295 (2007804)	2007804..2008148	+	345	WP_002378467.1	ImmA/IrrE family metallo-endopeptidase	Toxin
L5I18_RS10300 (2008184)	2008184..2008912	+	729	WP_002381717.1	potassium channel family protein	-
L5I18_RS10305 (2009012)	2009012..2010160	+	1149	WP_002381716.1	site-specific integrase	-
L5I18_RS10310 (2010188)	2010188..2010631	-	444	WP_002381715.1	competence type IV pilus minor pilin ComGD	-
L5I18_RS10315 (2010628)	2010628..2010903	-	276	WP_002356991.1	competence type IV pilus major pilin ComGC	-
L5I18_RS10320 (2010903)	2010903..2011949	-	1047	WP_047649127.1	competence type IV pilus assembly protein ComGB	-
L5I18_RS10325 (2011906)	2011906..2012874	-	969	WP_002401324.1	competence type IV pilus ATPase ComGA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1970853..2029009	58156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (22-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-63)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13712.62 Da
• Isoelectric Point: 5.5523

>T235026 WP_002378467.1 NZ_CP091889:2007804-2008148 [Enterococcus faecalis]
MKSIKELVEEYEVELVFAPINKRACYEPVKRIIFVNQNLSIEEQEESIFHEFKHVVSHSDYIELYKIPSFRNKMEAEADY
HMFKCLIEKHDGQFNYSNVITHYNLKMGQETYLN

Nucleotide

• Length: 345 bp

>T235026 NZ_LR134193:c1524552-1524376 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGGTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12691.16 Da
• Isoelectric Point: 4.6307

>AT235026 WP_002364355.1 NZ_CP091889:2007455-2007787 [Enterococcus faecalis]
MNLLERIKKLAKDRDVSIYQLEEEINIGRNTIYQWNKRTPSSDKLEAVANYFNVSVDYLLGRTENPNQAGAKPSDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT235026 NZ_LR134193:1524253-1524308 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• AlphaFold DB: S4BLV4