Detailed information of TA system TA235012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2826392..2826963
• Replicon: chromosome
• Accession: NZ_CP091886
• Organism: Enterococcus faecalis strain 143-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L5I19_RS13780
• Protein ID: WP_002354774.1
• Coordinates: 2826392..2826733 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: L5I19_RS13785
• Protein ID: WP_002354773.1
• Coordinates: 2826733..2826963 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I19_RS13775 (2822407)	2822407..2826021	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
L5I19_RS13780 (2826392)	2826392..2826733	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L5I19_RS13785 (2826733)	2826733..2826963	-	231	WP_002354773.1	hypothetical protein	Antitoxin
L5I19_RS13790 (2827137)	2827137..2827352	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
L5I19_RS13795 (2827491)	2827491..2828483	+	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
L5I19_RS13800 (2828650)	2828650..2829288	-	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
L5I19_RS13805 (2829973)	2829973..2831589	+	1617	WP_002378864.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T235012 WP_002354774.1 NZ_CP091886:c2826733-2826392 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T235012 NZ_LR134173:3027418-3028725 [Legionella cherrii]
ATGGTAAAAAAGAATCAAACTCTTTATGTCTTAATGAATGGCATTTTAGTAGGGATGCTTGAGAAAACAGCTCAGGGAGG
ATTGAGATTTACCTACCACCACGATTGGTTAACTCAGGCAGGATCTCGGCCTATTTCGCTCTCTTTACCACTTATTAATC
CCACTTATTCTGGCGATGTAGTATATAATTTTTTTGATAACTTGCTTCCAGATAATCAGCAGATTCGTGCGCGCATTCAA
GCACGCTTTCAAATAGCGACCAGCCAGCCCTTTGATCTGCTCGCGGGTATTGGTAGAGATTGCGTAGGAGCCATTCAGCT
GGTAACGGCTCCATTAGTTGAATTTGAGAAAAAAATTAAATTTAAACCTGTAAGTGAGCAAAAAATTGCCTCAATTTTAC
GAAATTATCGAATAAACCCCTTAGGCATGAGCCAAGAGGACGAAGACTTCCGAATTTCTATTGCAGGAGCGCAAGAAAAA
ACAGCTTTTTTATATCATGAAAACCAATGGTGTGAACCACTTCAAGAAACGCCAACCACCCATATCTTTAAATTACCCAT
TGGTGTTATTGCTCACCAGCAAATGGATTTAAGCGACAGTTGCGAAAACGAGTGGCTATGTTCCCTTCTTGCGAAAAGCT
ATGGACTACCAGTAGCCGATTGCAACATTCTTCATTTTGAAGACGTAAAGGTTTTATCTGTAAAACGATTTGATCGCAGA
CTGTCTCGCGATAAAAGCTGGATTATGAGATTGCCTCAAGAGGATATGTGCCAAGCACTGGGCGTTTCATACAACATTAA
GTATCAATCGGATGGAGGCCCAGGTATAAAAGAAATTATGAACTTATTACTTGGGTCAGCAAACTCAATTGCCGATCGGG
ATATGTTTTATCGAGCCCAGGTTTTTTTCTGGTTACTTGCCGCAATTGATGGACATGCTAAAAATTTCAGCCTTTTTATT
GAGGCTGAAGGAAAATACAGGCTCTCACCATTATATGACATTATCTCCGCATATCCCTTGATCAAGAAAAATCAACTGCA
ATCCCAGAAAATTAAAATGGCTATGGCTTTAAAGGGAAAAAACAATCATTACCATTGGCATACCTTGCAACGTCGACATT
TTTTAGACACGTCAAAAGACGCCAATTACTCTTCGGACCGCGCTGAAATGATTCTCAATGACATGTTGGAAAAAACAGAT
TCAGTGATACAAGAAGTCTCAAAAAAATTACCTGCTGAATTTCCAAATCATATTTCTGAGCCTATATTTGAGGGTTTGCA
TAAAGCAAAGCAAAAATTAGAACAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT235012 WP_002354773.1 NZ_CP091886:c2826963-2826733 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT235012 NZ_LR134173:3027176-3027424 [Legionella cherrii]
ATGCTGATCCACTCACCACAGGAATTGGCACTGTTGATAAAAAATCAACGAAAAAAACTAAAACTAAGCCAAGCTGAAGT
TGCTGATTTAGTAGGCTTAAAGCAAAAAACCATTTCTGCCATTGAAAATAATCCCGAAAATATGAGATTAAGTACTTTAT
TTCGAATATTATCTGCTCTTAGTCTAGATTTCAAAGCATCAACAAAAAGCGAACAAACAACAACCAATCCATGGAATGAT
GAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812