Detailed information of TA system TA235003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 426446..426980
• Replicon: chromosome
• Accession: NZ_CP091886
• Organism: Enterococcus faecalis strain 143-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: L5I19_RS02190
• Protein ID: WP_002360769.1
• Coordinates: 426705..426980 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: L5I19_RS02185
• Protein ID: WP_002369771.1
• Coordinates: 426446..426712 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I19_RS02150 (422496)	422496..423089	+	594	WP_002414802.1	replication-relaxation family protein	-
L5I19_RS02155 (423095)	423095..423583	+	489	WP_010710133.1	hypothetical protein	-
L5I19_RS02160 (424175)	424175..424441	+	267	WP_002377947.1	hypothetical protein	-
L5I19_RS02165 (424474)	424474..424974	+	501	WP_002360775.1	DnaJ domain-containing protein	-
L5I19_RS02170 (424987)	424987..425235	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
L5I19_RS02175 (425311)	425311..425727	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
L5I19_RS02180 (425751)	425751..426335	+	585	WP_238458177.1	thermonuclease family protein	-
L5I19_RS02185 (426446)	426446..426712	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
L5I19_RS02190 (426705)	426705..426980	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
L5I19_RS02195 (427171)	427171..427815	-	645	WP_002377952.1	IS6 family transposase	-
L5I19_RS02200 (428039)	428039..429013	+	975	WP_002355428.1	choloylglycine hydrolase	-
L5I19_RS02205 (429682)	429682..430290	-	609	Protein_411	IS6 family transposase	-
L5I19_RS02210 (430322)	430322..430507	-	186	WP_002358660.1	hypothetical protein	-
L5I19_RS02215 (430537)	430537..430866	+	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
L5I19_RS02220 (430994)	430994..431932	+	939	WP_002370935.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	EF0485 / bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / esp / psaA	398594..521848	123254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T235003 WP_002360769.1 NZ_CP091886:426705-426980 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T235003 NZ_LR134169:1567075-1567326 [Actinobacillus lignieresii]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACAATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATAGTTCACTTGAAGAAACCGCCTATT
TAATGCGTTCCCCGGCTAACGCTCAAAGATTACTAGACAGCATGGCAGAATTGAAGCAAGGGAAAGGCATTGAAAGAGAG
CTAATCGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT235003 WP_002369771.1 NZ_CP091886:426446-426712 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT235003 NZ_LR134169:1567323-1567583 [Actinobacillus lignieresii]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGTCTGATTAAAGATATTCAACGCCAACCATTCGAAGGTATAGGAAAGCCTGAACCGCTAAAATTCAATCTTTCGG
GCTTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACACCGTGGAAGATGAAACAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7