Detailed information of TA system TA235000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 121142..122161
• Replicon: chromosome
• Accession: NZ_CP091886
• Organism: Enterococcus faecalis strain 143-1

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: Q839N5
• Locus tag: L5I19_RS00520
• Protein ID: WP_002359283.1
• Coordinates: 121142..121780 (-)
• Length: 213 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: L5I19_RS00525
• Protein ID: WP_002359284.1
• Coordinates: 121787..122161 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I19_RS00495 (117059)	117059..118327	+	1269	WP_002356139.1	RidA family protein	-
L5I19_RS00500 (118377)	118377..118820	-	444	WP_002356140.1	MepB family protein	-
L5I19_RS00505 (118835)	118835..119380	-	546	WP_002401626.1	ATP:cob(I)alamin adenosyltransferase	-
L5I19_RS00510 (119531)	119531..119827	-	297	WP_002391749.1	hypothetical protein	-
L5I19_RS00515 (120525)	120525..121100	+	576	WP_002359282.1	restriction endonuclease	-
L5I19_RS00520 (121142)	121142..121780	-	639	WP_002359283.1	ImmA/IrrE family metallo-endopeptidase	Toxin
L5I19_RS00525 (121787)	121787..122161	-	375	WP_002359284.1	helix-turn-helix domain-containing protein	Antitoxin
L5I19_RS00530 (123003)	123003..123185	-	183	WP_002359286.1	hypothetical protein	-
L5I19_RS00535 (123352)	123352..123603	+	252	WP_002359287.1	hypothetical protein	-
L5I19_RS00540 (123690)	123690..124016	+	327	WP_002359288.1	hypothetical protein	-
L5I19_RS00545 (124016)	124016..124423	+	408	WP_002359289.1	DUF961 family protein	-
L5I19_RS00550 (124790)	124790..126109	-	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-
L5I19_RS00555 (126512)	126512..126703	+	192	WP_010773668.1	hypothetical protein	-
L5I19_RS00560 (126685)	126685..127089	+	405	WP_002359292.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	EF0149 / tufA	114674..193598	78924
	-	flank	IS/Tn	-	-	124790..126109	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (86-176)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 213 a.a.
• Molecular weight: 25186.21 Da
• Isoelectric Point: 9.1676

>T235000 WP_002359283.1 NZ_CP091886:c121780-121142 [Enterococcus faecalis]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDMPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILLK

Nucleotide

• Length: 639 bp

>T235000 NZ_LR134168:c1374560-1374261 [Haemophilus influenzae]
ATGACAATCCAAATCAAAACCACTCTGACATTTGATTCTTGGTTAAGCAAACTAAAAAACTTGCGTGCCAAAGCGAAAAT
AAACGCACGAATTAAACGCTTACAGTTCGGCAACTTTGGTGATATCAAAAGTGTGAATGATGGGATTTTTGAATTACGGA
TTGATGAAGGTCAAGGTTATCGAATTTATCTTAAAAACCAGAATGGCGTATTAGTGATTTTACTTTGTGGCGGAGATAAA
TCCACACAAGAGAAAGATATTAAACAAGCAAAACTTCTCGCACAGGAGCTAGGATTATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14518.29 Da
• Isoelectric Point: 4.8896

>AT235000 WP_002359284.1 NZ_CP091886:c122161-121787 [Enterococcus faecalis]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE

Nucleotide

• Length: 375 bp

>AT235000 NZ_LR134168:c1374264-1373971 [Haemophilus influenzae]
ATGACTGAACAATTAAAAGACTTTGATGTGGCAGAACACCTCATTTCTGAAGAAGAAATTCAACTTTACCTTAATGAAAT
TCTACAAGAAGATAATATTGAGCTTATTTTATCCTCCCTTGGCGACATAGCTCGTGCGCGTAACATGAGCCAAATCGCAC
GTGATGCAGGGATAAGCCGAGAAGGTCTTTATAAAGCCTTATCTGGCACGGGCAATCCTACTTTTGCTACTGTAATGAAA
GTAATGAAAGCCTTAAATTTACAGTTCCAAGTTCAACAATCTCGATTCGCCTAA

Structures

Toxin

• AlphaFold DB: A0A059N6D0