Detailed information of TA system TA234986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1532137..1533274
• Replicon: chromosome
• Accession: NZ_CP091884
• Organism: Enterococcus faecalis strain 152

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: L5I24_RS07505
• Protein ID: WP_002401483.1
• Coordinates: 1532137..1533000 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: L5I24_RS07510
• Protein ID: WP_000301765.1
• Coordinates: 1533002..1533274 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5I24_RS07480 (1527524)	1527524..1528291	-	768	WP_002357481.1	ribonuclease HII	-
L5I24_RS07485 (1528293)	1528293..1529144	-	852	WP_002357480.1	ribosome biogenesis GTPase YlqF	-
L5I24_RS07490 (1529339)	1529339..1530019	-	681	WP_048952191.1	IS6-like element IS1216 family transposase	-
L5I24_RS07495 (1530094)	1530094..1531170	+	1077	Protein_1450	IS1380-like element ISSsu5 family transposase	-
L5I24_RS07500 (1531379)	1531379..1531696	-	318	WP_002333463.1	hypothetical protein	-
L5I24_RS07505 (1532137)	1532137..1533000	-	864	WP_002401483.1	zeta toxin family protein	Toxin
L5I24_RS07510 (1533002)	1533002..1533274	-	273	WP_000301765.1	antitoxin	Antitoxin
L5I24_RS07515 (1533291)	1533291..1533506	-	216	WP_002295735.1	peptide-binding protein	-
L5I24_RS07520 (1533605)	1533605..1534501	-	897	WP_002387620.1	ParA family protein	-
L5I24_RS07525 (1534604)	1534604..1534864	-	261	Protein_1456	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
L5I24_RS07530 (1535034)	1535034..1535771	-	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
L5I24_RS07535 (1535896)	1535896..1535979	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
L5I24_RS07540 (1536048)	1536048..1536170	-	123	Protein_1459	peptide-binding protein	-
L5I24_RS07545 (1536290)	1536290..1537816	-	1527	WP_025191980.1	type IA DNA topoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / aph(3')-III / ant(6)-Ia / aac(6')-aph(2'')	-	1529339..1548352	19013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T234986 WP_002401483.1 NZ_CP091884:c1533000-1532137 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T234986 NZ_LR134165:c1461597-1460851 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACATGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGATGACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT234986 WP_000301765.1 NZ_CP091884:c1533274-1533002 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT234986 NZ_LR134165:c1460851-1460678 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3