Detailed information of TA system TA234976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4493396..4494654
• Replicon: chromosome
• Accession: NZ_CP091880
• Organism: Pseudomonas aeruginosa strain US449

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: L8E65_RS20900
• Protein ID: WP_003112528.1
• Coordinates: 4493396..4494151 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: L8E65_RS20905
• Protein ID: WP_003086329.1
• Coordinates: 4494286..4494654 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8E65_RS20890 (L8E65_20875)	4488568..4491111	+	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-
L8E65_RS20895 (L8E65_20880)	4491266..4492627	-	1362	WP_079262974.1	DNA recombination protein RmuC	-
L8E65_RS20900 (L8E65_20885)	4493396..4494151	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
L8E65_RS20905 (L8E65_20890)	4494286..4494654	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
L8E65_RS20910 (L8E65_20895)	4494727..4496013	-	1287	WP_034071738.1	FAD-binding oxidoreductase	-
L8E65_RS20915 (L8E65_20900)	4496069..4497562	-	1494	WP_003086325.1	aldehyde dehydrogenase family protein	-
L8E65_RS20920 (L8E65_20905)	4497737..4498213	+	477	WP_003112531.1	hypothetical protein	-
L8E65_RS20925 (L8E65_20910)	4498216..4498491	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T234976 WP_003112528.1 NZ_CP091880:c4494151-4493396 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T234976 NZ_LR134163:c3598472-3598269 [Yersinia pseudotuberculosis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT234976 WP_003086329.1 NZ_CP091880:c4494654-4494286 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT234976 NZ_LR134163:c3598889-3598521 [Yersinia pseudotuberculosis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7