Detailed information of TA system TA234975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3581033..3582254
• Replicon: chromosome
• Accession: NZ_CP091880
• Organism: Pseudomonas aeruginosa strain US449

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L8E65_RS16610
• Protein ID: WP_003162864.1
• Coordinates: 3581676..3582254 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L8E65_RS16605
• Protein ID: WP_003088112.1
• Coordinates: 3581033..3581587 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L8E65_RS16580 (L8E65_16565)	3576601..3577152	+	552	WP_057393193.1	XRE family transcriptional regulator	-
L8E65_RS16585 (L8E65_16570)	3577149..3577547	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
L8E65_RS16590 (L8E65_16575)	3577560..3577883	-	324	WP_023875494.1	multidrug efflux SMR transporter	-
L8E65_RS16595 (L8E65_16580)	3578214..3578675	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
L8E65_RS16600 (L8E65_16585)	3578680..3580875	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
L8E65_RS16605 (L8E65_16590)	3581033..3581587	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
L8E65_RS16610 (L8E65_16595)	3581676..3582254	+	579	WP_003162864.1	HD domain-containing protein	Toxin
L8E65_RS16615 (L8E65_16600)	3582362..3583549	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
L8E65_RS16620 (L8E65_16605)	3583539..3585710	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
L8E65_RS16625 (L8E65_16610)	3585700..3586977	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21911.78 Da
• Isoelectric Point: 5.3655

>T234975 WP_003162864.1 NZ_CP091880:3581676-3582254 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDILRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T234975 NZ_LR134163:2442413-2442781 [Yersinia pseudotuberculosis]
ATGCAGATCTTACCTGTTACTCCGAAACGGGCGGCTTATGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAACAGCATTACGGATTGGCTCTTAACGATACCGAGTTCAGTGATGACGCCGTGATCCACGAATATATTG
ATGCTGGTATCTCTCTGTCCGATGCGTTGAATTTCACGGTGGAAAAGTTTGGCCTGGTTCGTATCGATCGCCGGGGGTTC
TCCTGCCAGGAACAATCTCCCTTCATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCCACCGGCCTGATGACCCGTCT
GGGTTACCAGACCATCATCTCAGTTATCCGGGGCGAAAAGCAGACATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT234975 WP_003088112.1 NZ_CP091880:3581033-3581587 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT234975 NZ_LR134163:2442025-2442360 [Yersinia pseudotuberculosis]
ATGCCATCAATCACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGTCTGGTACAGGAAGG
CGACCGACTACATTTTCTAGCCGACCGTGCCGATATCAACGGAACCTTCAGCGAAGTGCAGGTACGTGATTTAGATAACG
CCTTCCCGCAGTTCATCAACTACCTGGAACTGATGCTGCTCTCCGGTGAGCTCAACCCTCGCCACCAGCACTGTGTCACG
TTGTATCGCAATGGCTTGACCTGTGAGGCCGATAGCCTGGGATCACACGGTTATGTGTATCTCGCTATTTATCCTACTCC
CCAATCAACTGCATAA