Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 1853640..1855148 Replicon chromosome
Accession NZ_CP091880
Organism Pseudomonas aeruginosa strain US449

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag L8E65_RS08635 Protein ID WP_003119682.1
Coordinates 1853640..1854227 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1C7C4U7
Locus tag L8E65_RS08640 Protein ID WP_003091607.1
Coordinates 1854294..1855148 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L8E65_RS08630 (L8E65_08625) 1849895..1853374 - 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
L8E65_RS08635 (L8E65_08630) 1853640..1854227 + 588 WP_003119682.1 GNAT family protein Toxin
L8E65_RS08640 (L8E65_08635) 1854294..1855148 + 855 WP_003091607.1 AraC family transcriptional regulator Antitoxin
L8E65_RS08645 (L8E65_08640) 1855245..1857410 + 2166 WP_003091606.1 TonB-dependent receptor family protein -
L8E65_RS08650 (L8E65_08645) 1857585..1859459 + 1875 WP_003117607.1 MFS transporter -
L8E65_RS08655 (L8E65_08650) 1859520..1859729 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T234974 WP_003119682.1 NZ_CP091880:1853640-1854227 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T234974 NZ_LR134163:c1366198-1365773 [Yersinia pseudotuberculosis]
GTGGCCCAGTGGCGATGTAACCTGCGGGTATCCTGGCATACTCAACTCTTTTCTTTATTGGCACACGGCATATTGGTCAT
TTTAACCTTGGTCGCGCCCTGGCCCTTGGGTTATACCGCCCTATGGCTGGTATTGCTGACGTTAGTGGTGTTTGAGTGTA
TCCGCAGCCAGAAAAGGATTAAATCCTGTCAGGGGGAAATCAGGCTAAAACCTGGCAACCTGGTGCTATGGAAAAGGCAT
GAATGGACCGTGGTCAAGCCGCCGTGGATCACCCGCTATGGGGTATTATTACATTTACAACAAACAGGCGGCCACGCGAC
CCGAAAGCGTCTATGGCTGTCCGCTGACAGCATGTCTGAAGATGAGTGGCGGCAGCTATGCCTGTTGCTGCGGCATTCAT
TTGAATCTGACGATGGAACGATGTGA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31895.20 Da        Isoelectric Point: 7.5295

>AT234974 WP_003091607.1 NZ_CP091880:1854294-1855148 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Download         Length: 855 bp

>AT234974 NZ_LR134163:c1366445-1366179 [Yersinia pseudotuberculosis]
ATGGAAATTGATAATAAAGCCCGTATTCACTGGGCATGCCGCCGTGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
TTTTGAATATGAATATGATAGCTTGAGCGATAACGAGAAACAGGCATTTATCCGCTTGCTTGAGTGTGATGACCCAGATT
TATTCAACTGGCTGATGAACCACGGGGAGCCGCAGGATAGCGAGCTATATCAGATGGTCAAACTGATTCAATCACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C4U7

References