Detailed information of TA system TA234941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 700573..701102
• Replicon: chromosome
• Accession: NZ_CP091876
• Organism: Staphylococcus aureus strain FRI-1169

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MAK06_RS03535
• Protein ID: WP_000621175.1
• Coordinates: 700740..701102 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: MAK06_RS03530
• Protein ID: WP_000948331.1
• Coordinates: 700573..700743 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAK06_RS03500 (695610)	695610..696170	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
MAK06_RS03505 (696378)	696378..696857	+	480	WP_001287088.1	hypothetical protein	-
MAK06_RS03510 (696850)	696850..698433	+	1584	WP_238097444.1	PH domain-containing protein	-
MAK06_RS03515 (698420)	698420..698911	+	492	WP_001205910.1	PH domain-containing protein	-
MAK06_RS03520 (698915)	698915..699274	+	360	WP_000581200.1	holo-ACP synthase	-
MAK06_RS03525 (699340)	699340..700488	+	1149	WP_001281145.1	alanine racemase	-
MAK06_RS03530 (700573)	700573..700743	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MAK06_RS03535 (700740)	700740..701102	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAK06_RS03540 (701451)	701451..702452	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
MAK06_RS03545 (702571)	702571..702897	+	327	WP_001052491.1	anti-sigma factor antagonist	-
MAK06_RS03550 (702899)	702899..703378	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
MAK06_RS03555 (703353)	703353..704123	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T234941 WP_000621175.1 NZ_CP091876:700740-701102 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T234941 NZ_LR134152:711885-712181 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT234941 WP_000948331.1 NZ_CP091876:700573-700743 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT234941 NZ_LR134152:712183-712578 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1