Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2283049..2283578 Replicon chromosome
Accession NZ_CP091875
Organism Staphylococcus aureus strain MN8

Toxin (Protein)


Gene name mazF Uniprot ID Q6GF05
Locus tag MAK07_RS11475 Protein ID WP_000621176.1
Coordinates 2283049..2283411 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID T1YCG8
Locus tag MAK07_RS11480 Protein ID WP_000948331.1
Coordinates 2283408..2283578 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAK07_RS11455 (2280028) 2280028..2280798 - 771 WP_001041107.1 RNA polymerase sigma factor SigB -
MAK07_RS11460 (2280773) 2280773..2281252 - 480 WP_001190825.1 anti-sigma B factor RsbW -
MAK07_RS11465 (2281254) 2281254..2281580 - 327 WP_001052491.1 anti-sigma factor antagonist -
MAK07_RS11470 (2281699) 2281699..2282700 - 1002 WP_000390829.1 PP2C family protein-serine/threonine phosphatase -
MAK07_RS11475 (2283049) 2283049..2283411 - 363 WP_000621176.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MAK07_RS11480 (2283408) 2283408..2283578 - 171 WP_000948331.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MAK07_RS11485 (2283663) 2283663..2284811 - 1149 WP_001281140.1 alanine racemase -
MAK07_RS11490 (2284877) 2284877..2285236 - 360 WP_000581197.1 holo-ACP synthase -
MAK07_RS11495 (2285240) 2285240..2285731 - 492 WP_001286801.1 PH domain-containing protein -
MAK07_RS11500 (2285724) 2285724..2287301 - 1578 WP_001294646.1 PH domain-containing protein -
MAK07_RS11505 (2287294) 2287294..2287773 - 480 WP_001287082.1 hypothetical protein -
MAK07_RS11510 (2287982) 2287982..2288542 - 561 WP_001092414.1 K(+)-transporting ATPase subunit C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13469.74 Da        Isoelectric Point: 10.1654

>T234925 WP_000621176.1 NZ_CP091875:c2283411-2283049 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVVHQKN

Download         Length: 363 bp

>T234925 NZ_LR134151:c919127-918837 [Serratia plymuthica]
GTGCCACAACTGATATGGACGCCAGCGGCGCTGCTGGATGTACAGCGGCTTTATCGATTTTTGGCTGAGAAGGATCTCAA
TTCGGCAAAGCGCGCTGTCGCCACTATTCGTAACAGCATCAAAATAATAGCGCACCAACCCCATATTGGCCGTCCCGCTA
AAGATATGGCACCTGAATTCAGGGAGTGGCCCGTCGATTTCGGCAATAGCGGTTATCTTGTTATGTATCGGTTTAACGGT
GTAACCGCCGTTATTTTGGCCATTCGCCACCAAAGTGAAACCGGGTATTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6251.78 Da        Isoelectric Point: 3.8305

>AT234925 WP_000948331.1 NZ_CP091875:c2283578-2283408 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Download         Length: 171 bp

>AT234925 NZ_LR134151:c919393-919115 [Serratia plymuthica]
ATGGCTACTGCTACGTCGATAAAAATTGATGATGAGCTTAAGCAACGGGTGCAACACATTGCCGCTACCCGCCAACGCAG
CGCACACTGGATTATGCGTGAAGCTATTCGTGAATATGTCGAGCGTGAAGAAAAACGCGAGGCTGTTCGCAACGATGCAT
TGCAGGCATGGGAAACCTATCAGGCCAATGGTAAACATTTGACTCAGGACGAGGCCGACGGTTGGCTCTCCGATCTGGAA
AAAGGTGACGATACGGAGATACCCGGGTGCCACAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A168PYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0VRZ1

References