Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /HTH_19(antitoxin)
Location 2097735..2098523 Replicon chromosome
Accession NZ_CP091875
Organism Staphylococcus aureus strain MN8

Toxin (Protein)


Gene name - Uniprot ID A0A2I7Y5B3
Locus tag MAK07_RS10380 Protein ID WP_000525004.1
Coordinates 2098062..2098523 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag MAK07_RS10375 Protein ID WP_000333630.1
Coordinates 2097735..2098049 (+) Length 105 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MAK07_RS10320 (2093142) 2093142..2093765 - 624 WP_000139720.1 DUF1071 domain-containing protein -
MAK07_RS10325 (2093758) 2093758..2093979 - 222 WP_001077280.1 DUF2483 family protein -
MAK07_RS10330 (2093989) 2093989..2094249 - 261 WP_000291090.1 DUF1108 family protein -
MAK07_RS10335 (2094346) 2094346..2094507 - 162 WP_000066011.1 DUF1270 domain-containing protein -
MAK07_RS10340 (2094500) 2094500..2094721 - 222 WP_000594789.1 hypothetical protein -
MAK07_RS10345 (2094792) 2094792..2095022 + 231 WP_000395455.1 hypothetical protein -
MAK07_RS10350 (2095198) 2095198..2095938 - 741 WP_001148589.1 phage antirepressor KilAC domain-containing protein -
MAK07_RS10355 (2095940) 2095940..2096125 - 186 WP_000933365.1 helix-turn-helix transcriptional regulator -
MAK07_RS10360 (2096571) 2096571..2097053 + 483 WP_000394410.1 hypothetical protein -
MAK07_RS10365 (2097154) 2097154..2097333 - 180 WP_000438352.1 hypothetical protein -
MAK07_RS10370 (2097347) 2097347..2097583 - 237 WP_001121116.1 helix-turn-helix transcriptional regulator -
MAK07_RS10375 (2097735) 2097735..2098049 + 315 WP_000333630.1 helix-turn-helix transcriptional regulator Antitoxin
MAK07_RS10380 (2098062) 2098062..2098523 + 462 WP_000525004.1 hypothetical protein Toxin
MAK07_RS10385 (2098707) 2098707..2099639 + 933 WP_000392183.1 hypothetical protein -
MAK07_RS10390 (2099619) 2099619..2099798 - 180 WP_000337827.1 hypothetical protein -
MAK07_RS10395 (2099911) 2099911..2100957 + 1047 WP_001145722.1 tyrosine-type recombinase/integrase -
MAK07_RS10400 (2101002) 2101002..2102147 + 1146 WP_001245443.1 radical SAM/CxCxxxxC motif protein YfkAB -
MAK07_RS10405 (2102407) 2102407..2102937 - 531 WP_000184383.1 acyl-CoA thioesterase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - hysA / selk 1946529..2146601 200072


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(4-62)


Sequences


Toxin        


Download         Length: 154 a.a.        Molecular weight: 18074.46 Da        Isoelectric Point: 4.6915

>T234920 WP_000525004.1 NZ_CP091875:2098062-2098523 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T234920 NZ_LR134139:c2478511-2478269 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAGACATTTATAGAAATTGTTGAGACTTT
AAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATTTAGAGCGATATTCAAGAAGAATTA
ACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATACTATCGGCATGGTCACATTATGAT
TAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT234920 WP_000333630.1 NZ_CP091875:2097735-2098049 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT234920 NZ_LR134139:c2478768-2478511 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2I7Y5B3


Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References