Detailed information of TA system TA234904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1332364..1333280
• Replicon: chromosome
• Accession: NZ_CP091872
• Organism: Bacillus sp. Man122

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: MAK48_RS06995
• Protein ID: WP_003244695.1
• Coordinates: 1332534..1333280 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: MAK48_RS06990
• Protein ID: WP_003232646.1
• Coordinates: 1332364..1332534 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAK48_RS06955 (1329227)	1329227..1329556	+	330	WP_003232660.1	XkdW family protein	-
MAK48_RS06960 (1329553)	1329553..1329717	+	165	WP_003232658.1	XkdX family protein	-
MAK48_RS06965 (1329761)	1329761..1330600	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
MAK48_RS06970 (1330653)	1330653..1330922	+	270	WP_003232655.1	hemolysin XhlA family protein	-
MAK48_RS06975 (1330935)	1330935..1331198	+	264	WP_003232653.1	phage holin	-
MAK48_RS06980 (1331211)	1331211..1332104	+	894	WP_029317679.1	N-acetylmuramoyl-L-alanine amidase	-
MAK48_RS06985 (1332141)	1332141..1332278	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MAK48_RS06990 (1332364)	1332364..1332534	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MAK48_RS06995 (1332534)	1332534..1333280	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MAK48_RS07000 (1333390)	1333390..1334391	-	1002	WP_003232642.1	inorganic phosphate transporter	-
MAK48_RS07005 (1334404)	1334404..1335021	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MAK48_RS07010 (1335297)	1335297..1336613	-	1317	WP_003232640.1	serine/threonine exchanger	-
MAK48_RS07015 (1337002)	1337002..1337952	+	951	WP_003232637.1	VOC family protein	-
MAK48_RS07020 (1338061)	1338061..1338156	+	96	Protein_1319	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T234904 WP_003244695.1 NZ_CP091872:c1333280-1332534 [Bacillus sp. Man122]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T234904 NZ_LR134136:c1186245-1186027 [Atlantibacter hermannii]
ATGTCAGAAAAACCCTTAACGAAAACTGATTATTTAATGCGTCTACGACGCTGCCAGACTATTGATACGCTTGAAAGAGT
CATTGAAAAAAATAAATACGAATTATCGGATAACGAATTGGCGGTATTTTATTCAGCGGCCGATCACCGTCTTGCCGAGC
TCACAATGAATAAGCTTTACGATAAAATACCATCGTCTGTGTGGAAATTTATTCGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT234904 WP_003232646.1 NZ_CP091872:c1332534-1332364 [Bacillus sp. Man122]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT234904 NZ_LR134136:c1186648-1186274 [Atlantibacter hermannii]
ATGGACGAATACTCACCAAAACGGCATGATATAGCCCAGCTAAAATTCCTGTGTGAAAATCTCTATCATGACTGCCTGCT
AACCCTTGGTGACAGCAATCATGGTTGGGTGAACGATCCAACCTCGGCCATCAACCTGCAGTTAAATGAATTGATTGAGC
ATATTGCGAGTTTTGCGCTTAATTATAAAATTAAGCATGACGAAGATAATGCATTGATCGAACACGTTGATGAGTATCTT
GATGATACCTTCATGCTGTTCAGCAACTATGGTATTAGCGCTCAGGATTTACAGAAATGGCGCAAATCAGGAAACCGTTT
GTTTCGATGTTTCGTCAACGTCAGTAAGGCCAACCCAGTGAGCCTATCTTTCTAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8