Detailed information of TA system TA234903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518374..519010
• Replicon: chromosome
• Accession: NZ_CP091872
• Organism: Bacillus sp. Man122

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: MAK48_RS02645
• Protein ID: WP_003156187.1
• Coordinates: 518660..519010 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: MAK48_RS02640
• Protein ID: WP_003225183.1
• Coordinates: 518374..518655 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAK48_RS02620 (514733)	514733..515332	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
MAK48_RS02625 (515427)	515427..515792	+	366	WP_003234281.1	holo-ACP synthase	-
MAK48_RS02630 (515958)	515958..516974	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
MAK48_RS02635 (517089)	517089..518258	+	1170	WP_003234284.1	alanine racemase	-
MAK48_RS02640 (518374)	518374..518655	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
MAK48_RS02645 (518660)	518660..519010	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
MAK48_RS02650 (519125)	519125..519949	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
MAK48_RS02655 (519954)	519954..520319	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
MAK48_RS02660 (520323)	520323..520724	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
MAK48_RS02665 (520736)	520736..521743	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
MAK48_RS02670 (521805)	521805..522134	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
MAK48_RS02675 (522131)	522131..522613	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
MAK48_RS02680 (522579)	522579..523367	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
MAK48_RS02685 (523367)	523367..523966	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T234903 WP_003156187.1 NZ_CP091872:518660-519010 [Bacillus sp. Man122]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T234903 NZ_LR134136:c970705-970391 [Atlantibacter hermannii]
ATGAAAACTTTACCTGCAACAACTCCGCAGGCAGCGAAGCTATGTCTGCCGCCCGTGGCTGTTTGGCAAATGTTACTGGC
ACGCCTGCTGGAACAGCACTATGGCCTGACACTGAACGATACATCGTTCAGTGATGAAACTGTTATTCAGGAACATATTG
ATGCCGGGATTACCCTGGCCGATGCAGTCAACTTTCTGGTGGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGGTTG
AGTTGGCAGGCGCAATCATCCTATCTTCGGGCTGTAGATATCCTACGAGCTCGACAGGCAACTGGGTTGTTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT234903 WP_003225183.1 NZ_CP091872:518374-518655 [Bacillus sp. Man122]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT234903 NZ_LR134136:c971043-970726 [Atlantibacter hermannii]
ATGAGCAACATCACATGGGGCCTGCAGCGGGATATTACGCCACGCCTGGGAGCTCGCCTTGTGCAGGAGGGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGTAAATTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATCGAGTCGATGCTGACCACCTATGAACTGAATCCCCGCCATGCCCACTGCGTCATCCTGTATCAC
AATGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACGTCGCCGTTTATCCCACTCAGCGCTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32