Detailed information of TA system TA234900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 125203..125724
• Replicon: plasmid unnamed1
• Accession: NZ_CP091857
• Organism: Rhodococcus erythropolis strain CERE8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: T5HL71
• Locus tag: ITJ47_RS01445
• Protein ID: WP_021334703.1
• Coordinates: 125419..125724 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ITJ47_RS01440
• Protein ID: WP_030538123.1
• Coordinates: 125203..125418 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITJ47_RS01415 (ITJ47_01415)	121074..121283	+	210	WP_166503019.1	hypothetical protein	-
ITJ47_RS01420 (ITJ47_01420)	121204..121506	-	303	Protein_130	transposase	-
ITJ47_RS01425 (ITJ47_01425)	121545..122858	-	1314	WP_030538128.1	IS256 family transposase	-
ITJ47_RS01430 (ITJ47_01430)	122996..123778	-	783	WP_021334706.1	DUF4145 domain-containing protein	-
ITJ47_RS01435 (ITJ47_01435)	124120..124839	+	720	WP_021334705.1	hypothetical protein	-
ITJ47_RS01440 (ITJ47_01440)	125203..125418	+	216	WP_030538123.1	hypothetical protein	Antitoxin
ITJ47_RS01445 (ITJ47_01445)	125419..125724	+	306	WP_021334703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ITJ47_RS01450 (ITJ47_01450)	125753..127720	+	1968	WP_238054054.1	hypothetical protein	-
ITJ47_RS01455 (ITJ47_01455)	127720..128517	+	798	WP_021334701.1	hypothetical protein	-
ITJ47_RS01460 (ITJ47_01460)	128643..128990	-	348	WP_021334700.1	PDDEXK nuclease domain-containing protein	-
ITJ47_RS01465 (ITJ47_01465)	129301..130359	+	1059	WP_165639875.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..232438	232438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 10515.97 Da
• Isoelectric Point: 4.7318

>T234900 WP_021334703.1 NZ_CP091857:125419-125724 [Rhodococcus erythropolis]
VRPIHDAQLGAGVSSVLVLTRELVRPHLGTVTVAPIRAEGRGLSTELSLGEGNGVEDGSVVDCDGLTTIQVSALGVNRGF
LLPAQEVLLTRALRSAFDLES

Nucleotide

• Length: 306 bp

>T234900 NZ_LR134117:2501536-2501961 [Serratia odorifera]
GTGGCCCAGTGGCGATGTAATGTCCGTATCTCCTGGCGCACCCAAATCGTTTCGCTGTTGGCGCACGGCGCGCTGATACT
GCTGATCCTGATTTCGCCGTGGCCCGAAGGTTACGGCCCGCTATGGCTGGTGCTGCTGACGCTGGTGGTGTTTGAATGCA
TCCGCAGCCAAAAGCGCATTGCCACGCGCCAGGGAGAGCTGCGGGTGCTGAGTGCGCCGCGGCATGTGGTGTGGGACGGC
CAGGAATGGCGGGTTATCGGCCGGCCATGGATGCCGCGCTTCGGTATTCTGTTCACGCTGGAACCGCTTGACGGGCAAAA
ACGACGTCGGCTGTGGCTGGCGTCCGACTGCATGAGCAAAGCGGACTGGCGGCAACTGCGCCAGCGGTTGTCGTTCCCGC
CGGAGGATGATAGCGAGGCGCCGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7525.58 Da
• Isoelectric Point: 10.4526

>AT234900 WP_030538123.1 NZ_CP091857:125203-125418 [Rhodococcus erythropolis]
MSTQIAVRLPEGIVAFLDGEVSGGRAKSRAQVVLRALEREQRRQLAVRDVEILSDGAGGDVNIAAAHGKKS

Nucleotide

• Length: 216 bp

>AT234900 NZ_LR134117:2501289-2501555 [Serratia odorifera]
ATGGATATCAATAACAAAGCACGTATTCACTGGGCTTGCCGCCGCGGCATGCGCGAACTGGATATCTCGATCATGCCGTT
TTTCGAATATGAGTACGATTCGTTGAGCGATAAAGACAAGGCGCTGTTTATTCGCCTGCTGGAATGTGACGATCCAGATT
TATTTAACTGGCTGATGAATCATGGCGCTCCGCAGGATGACGAACTGCAGAGAATGGTGACCTTGATCCAGACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTAA

Structures

Toxin

• AlphaFold DB: T5HL71